Mechanical stimulation (mechanotherapy) can promote skeletal muscle repair, but a lack of reproducible protocols and mechanistic understanding of the relation between mechanical cues and tissue regeneration limit progress in this field. To address these gaps, we developed a robotic device equipped with real-time force control and compatible with ultrasound imaging for tissue strain analysis. We investigated the hypothesis that specific mechanical loading improves tissue repair by modulating inflammatory responses that regulate skeletal muscle regeneration. We report that cyclic compressive loading within a specific range of forces substantially improves functional recovery of severely injured muscle in mice. This improvement is attributable in part to rapid clearance of neutrophil populations and neutrophil-mediated factors, which otherwise may impede myogenesis. Insights from this work will help advance therapeutic strategies for tissue regeneration broadly.

中文翻译：

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骨骼肌再生与机器人驱动介导的中性粒细胞清除

机械刺激（机械疗法）可以促进骨骼肌修复，但缺乏可重复的方案和对机械线索与组织再生之间关系的机械理解限制了该领域的进展。为了解决这些差距，我们开发了一种配备实时力控制并与超声成像兼容的机器人设备，用于组织应变分析。我们研究了特定机械负荷通过调节调节骨骼肌再生的炎症反应来改善组织修复的假设。我们报告说，在特定的力范围内进行循环压缩载荷可以显着改善小鼠严重受伤肌肉的功能恢复。这种改善部分归因于中性粒细胞群和中性粒细胞介导因子的快速清除，否则可能会阻碍肌生成。这项工作的见解将有助于广泛推进组织再生的治疗策略。