Systematic Assessment of Accessibility to the Surface of Staphylococcus aureus,ACS Chemical Biology

当前位置： X-MOL 学术 › ACS Chem. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Systematic Assessment of Accessibility to the Surface of Staphylococcus aureus
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2021-10-05 , DOI: 10.1021/acschembio.1c00604
Noel J Ferraro ₁ , Seonghoon Kim ₂ , Wonpil Im ₃ , Marcos M Pires ₁

Affiliation

Proteins from bacterial foes, antimicrobial peptides, and host immune proteins must navigate past a dense layer of bacterial surface biomacromolecules to reach the peptidoglycan (PG) layer of Gram-positive bacteria. A subclass of molecules (e.g., antibiotics with intracellular targets) also must permeate through the PG (in a molecular sieving manner) to reach the cytoplasmic membrane. Despite the biological and therapeutic importance of surface accessibility, systematic analyses in live bacterial cells have been lacking. We describe a live cell fluorescence assay that is robust, shows a high level of reproducibility, and reports on the permeability of molecules to and within the PG scaffold. Moreover, our study shows that teichoic acids impede the permeability of molecules of a wide range of sizes and chemical composition.

中文翻译：

金黄色葡萄球菌表面可及性的系统评估

来自细菌敌人、抗菌肽和宿主免疫蛋白的蛋白质必须穿过一层致密的细菌表面生物大分子，才能到达革兰氏阳性细菌的肽聚糖 (PG) 层。分子的一个亚类（例如，具有细胞内靶标的抗生素）也必须通过 PG（以分子筛分方式）渗透到细胞质膜。尽管表面可及性具有生物学和治疗重要性，但仍缺乏对活细菌细胞的系统分析。我们描述了一种强大的活细胞荧光测定法，显示出高水平的重现性，并报告了分子对 PG 支架及其内部的渗透性。此外，我们的研究表明，磷壁酸会阻碍各种尺寸和化学成分的分子的渗透性。

更新日期：2021-11-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11