The complete genomic sequence of a novel ilarvirus from Eleocharis dulcis, tentatively named "water chestnut virus A" (WCVA), was determined using next-generation sequencing (NGS) combined with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The three genomic RNA components of WCVA were 3578 (RNA1), 2873 (RNA2), and 2073 (RNA3) nucleotides long, with four predicted open reading frames containing conserved domains and motifs typical of ilarviruses. Phylogenetic analysis of each predicted protein consistently placed WCVA in subgroup 4 of the genus Ilarvirus, together with prune dwarf virus, viola white distortion associated virus, Fragaria chiloensis latent virus, and potato yellowing virus. The genetic distances and lack of serological reaction to antisera against other ilarviruses suggest that WCVA is a novel member of the genus.

采用新一代测序 ( NGS ) 结合逆转录聚合酶链式反应 (RT-PCR) 和快速扩增cDNA 结束 (RACE) PCR。WCVA 的三个基因组 RNA 成分是 3578 (RNA1)、2873 (RNA2) 和 2073 (RNA3) 核苷酸长，具有四个预测的开放阅读框，其中包含典型的鼠病毒的保守结构域和基序。每种预测蛋白质的系统发育分析始终将 WCVA 置于 Ilarvirus 属的第 4 亚组中，连同西梅矮化病毒、堇菜白色变形相关病毒、草莓潜伏病毒和马铃薯黄变病毒。遗传距离和对其他虫病毒的抗血清缺乏血清学反应表明 WCVA 是该属的新成员。