Evaluating the Distribution of Bacterial Natural Product Biosynthetic Genes across Lake Huron Sediment,ACS Chemical Biology

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Evaluating the Distribution of Bacterial Natural Product Biosynthetic Genes across Lake Huron Sediment
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2021-10-04 , DOI: 10.1021/acschembio.1c00653
Maryam Elfeki ₁ , Shrikant Mantri ₂ , Chase M Clark ₁ , Stefan J Green ₃ , Nadine Ziemert ₂ , Brian T Murphy ₁

Affiliation

Environmental microorganisms continue to serve as a major source of bioactive natural products (NPs) and as an inspiration for many other scaffolds in the toolbox of modern medicine. Nearly all microbial NP-inspired therapies can be traced to field expeditions to collect samples from the environment. Despite the importance of these expeditions in the search for new drugs, few studies have attempted to document the extent to which NPs or their corresponding production genes are distributed within a given environment. To gain insights into this, the geographic occurrence of NP ketosynthase (KS) and adenylation (A) domains was documented across 53 and 58 surface sediment samples, respectively, covering 59,590 square kilometers of Lake Huron. Overall, no discernible NP geographic distribution patterns were observed for 90,528 NP classes of nonribosomal peptides and polyketides detected in the survey. While each sampling location harbored a similar number of A domain operational biosynthetic units (OBUs), a limited overlap of OBU type was observed, suggesting that at the sequencing depth used in this study, no single location served as a NP “hotspot”. These data support the hypothesis that there is ample variation in NP occurrence between sampling sites and suggest that extensive sample collection efforts are required to fully capture the functional chemical diversity of sediment microbial communities on a regional scale.

中文翻译：

评估细菌天然产物生物合成基因在休伦湖沉积物中的分布

环境微生物继续作为生物活性天然产物 (NPs) 的主要来源，并作为现代医学工具箱中许多其他支架的灵感来源。几乎所有受微生物 NP 启发的疗法都可以追溯到从环境中收集样本的实地考察。尽管这些探险在寻找新药方面很重要，但很少有研究试图记录 NP 或其相应的生产基因在给定环境中的分布程度。为了深入了解这一点，分别在 53 个和 58 个表层沉积物样本中记录了 NP 酮合酶 (KS) 和腺苷酸化 (A) 域的地理分布，覆盖面积为 59,590 平方公里的休伦湖。总体而言，没有观察到 90 个可辨别的 NP 地理分布模式，调查中检测到 528 种 NP 类非核糖体肽和聚酮化合物。虽然每个采样位置都包含相似数量的 A 域可操作生物合成单元 (OBU)，但观察到 OBU 类型的重叠有限，这表明在本研究中使用的测序深度，没有单个位置可用作 NP“热点”。这些数据支持这样的假设，即采样点之间的 NP 发生率存在很大差异，并表明需要大量的样本收集工作才能在区域范围内充分捕捉沉积物微生物群落的功能化学多样性。表明在本研究中使用的测序深度，没有一个位置可以作为 NP“热点”。这些数据支持这样的假设，即采样点之间的 NP 发生率存在很大差异，并表明需要大量的样本收集工作才能在区域范围内充分捕捉沉积物微生物群落的功能化学多样性。表明在本研究中使用的测序深度，没有一个位置可以作为 NP“热点”。这些数据支持这样的假设，即采样点之间的 NP 发生率存在很大差异，并表明需要大量的样本收集工作才能在区域范围内充分捕捉沉积物微生物群落的功能化学多样性。

更新日期：2021-11-19

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