Polyunsaturated fatty acids (PUFAs), esterified to phospholipids, are susceptible to oxidation. They form oxidized phospholipids (OxPLs) by oxygenases or reactive oxygen species (ROS), or both. These OxPLs are associated with various diseases, such as atherosclerosis, pulmonary injuries, neurodegenerative diseases, cancer, and diabetes. Since many types of OxPLs seem to be generated in vivo, precise determination of their structural diversity is required to understand their potential structure-specific functions. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful method to quantitatively measure the structural diversity of OxPLs present in biological samples. This review outlines recent advances in analytical methods for OxPLs and their physiological relevance in health and diseases.

酯化为磷脂的多不饱和脂肪酸 (PUFA) 易于氧化。它们通过加氧酶或活性氧 (ROS) 或两者形成氧化磷脂 (OxPL)。这些 OxPL 与各种疾病有关，例如动脉粥样硬化、肺损伤、神经退行性疾病、癌症和糖尿病。由于似乎在体内生成了许多类型的 OxPL，因此需要精确确定其结构多样性以了解其潜在的结构特异性功能。液相色谱-串联质谱 (LC-MS/MS) 是定量测量生物样品中存在的 OxPL 结构多样性的强大方法。本综述概述了 OxPL 分析方法的最新进展及其与健康和疾病的生理相关性。