The sedimentary fill of the Danube Basin represents the northwestern part of the Central Paratethys Sea. The middle Miocene opening of the basin was associated with volcanic activity. The altered tuff to tuffite layers from the northwest part of the Danube Basin occurs within the NN5 Zone and are accompanied by volcanic sediments with andesite clasts. The biotite ⁴⁰Ar/³⁹Ar age of 13.23 ± 1.95 Ma from the Trakovice-4 (1401–1406 m) tuff to tuffite layer covers a large time span, and this is caused by the fine grain size and alterations to biotite crystals. Nevertheless, the age range thus obtained agrees with biostratigraphic ranking. Fresh amphiboles from the Madunice-3 (1200–1195 m) volcanic sandstone give a more accurate age of 13.83 ± 0.11 Ma, and date the maximum fall in sea level to the beginning of late Badenian (Serravallian). The comparison of age data obtained from sedimentological, biostratigraphic and ecological analyses documents the following: (1) major deposition and subsidence took place in the Blatné depression during the late Badenian (early Serravallian, 13.82–12.6 Ma); (2) although the beginning of late Badenian stage is marked by the Badenian Salinity Crisis, only siliciclastic deposition is recorded within the Danube Basin; (3) during the entire late Badenian stage, upwelling conditions (estuarine circulation) are linked with diversified microfossil associations and dysoxic periods; (4) Moreover, the ⁴⁰Ar/³⁹Ar age of 13.83 ± 0.11 Ma supports the employment of Globoturborotalita druryi as an index fossil for the onset of the late Badenian. Thus, the Danube Basin represents a location with a well recognised 13.8 Ma early/late Badenian (Langhian /Serravallian) boundary.

多瑙河盆地的沉积填充物代表了中央副特提斯海的西北部。盆地的中新世开口与火山活动有关。多瑙河盆地西北部的蚀变凝灰岩至凝灰岩层出现在 NN5 区内，并伴有安山岩碎屑的火山沉积物。 Trakovice-4（1401~1406 m）凝灰岩到凝灰岩层的黑云母⁴⁰ Ar/ ³⁹ Ar 年龄为 13.23 ± 1.95 Ma，时间跨度很大，这是由细粒度和黑云母晶体蚀变造成的。尽管如此，由此获得的年龄范围与生物地层排名一致。来自 Madunice-3 (1200–1195 m) 火山砂岩的新鲜角闪石给出了更准确的年龄为 13.83 ± 0.11 Ma，并将海平面最大下降日期确定为巴登阶晚期 (Serravallian) 初期。沉积学、生物地层学和生态分析获得的年龄数据的比较证明了以下内容：（1）巴登纪晚期（塞拉瓦阶早期，13.82-12.6Ma），Blatné凹陷发生了主要的沉积和沉降； (2)虽然晚巴登期的开始以巴登期盐度危机为标志，但多瑙河盆地内仅记录到硅质碎屑沉积； （3）整个巴登阶晚期，上升流条件（河口环流）与多样化的微化石组合和缺氧期有关； (4) 此外， 13.83 ± 0.11 Ma 的⁴⁰ Ar/ ³⁹ Ar 年龄支持将Globoturborotalita druryi作为晚巴登阶爆发的指示化石。因此，多瑙河盆地代表了一个具有公认的 13.8 Ma 早/晚巴登阶（朗吉阶/塞拉瓦阶）边界的位置。