The Candida antarctica lipase B (CALB) was embedded in the metal-organic framework, zeolitic imidazolate framework-8 (ZIF-8), and applied in the enzymatic synthesis of L-ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF-8 achieved the enzyme loading of 80 mg g⁻¹ with 11.3 U g⁻¹ (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L-ascorbic acid in a 10-batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L⁻¹ h⁻¹, which was higher than ever reported continuous ASP biosynthesis reactions.

中文翻译：

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使用南极假丝酵母脂肪酶 B 包埋金属有机框架高效酶法合成 L-抗坏血酸棕榈酸酯

南极假丝酵母脂肪酶B（CALB）包埋在金属有机骨架沸石咪唑酯骨架8（ZIF-8）中，首次应用于酶法合成L-抗坏血酸棕榈酸酯（ASP）。获得的CALB@ZIF-8的酶载量为80 mg g ^-1，单位活性为11.3 U g ^-1，活性恢复率为59.8%，固定化率为92.7%。在最佳条件下，在 10 批次反应中合成了L-抗坏血酸转化率超过 75.9% 的 ASP。随后在填充床生物反应器中进行 ASP 的连续合成，平均时空产率为 58.1 g L ^-1  h ^-1，这比以往报道的连续 ASP 生物合成反应要高。