A Citrus sinensis R2R3 MYB transcription factor (CsMYB96) has previously been shown to be strongly associated with the expression of many genes related to wax biosynthesis in the fruit. In this study, CsMYB96 was found to alleviate water loss by simultaneously regulating the expression of genes encoding plasma membrane intrinsic proteins (CsPIPs) and wax-related genes. Expression profiling indicated that CsPIP1;1 and CsPIP2;4 had high expression that was representative of other aquaporins, and they were down-regulated in the peel of post-harvest citrus fruit. CsPIP2;4 was further characterized as the predominant CsPIP, with high expression and high-water channel activity. Transient overexpression of CsPIP2;4 accelerated water loss in citrus fruit. In silico analysis further indicated that the expression of CsMYB96 had a significant negative correlation with that of CsPIPs. In vivo and in vitro experiments confirmed that CsMYB96 was able to directly repress the expression of CsPIPs. In addition, CsMYB96 was able to activate wax-related genes and promote wax biosynthesis for defense against water loss. Transient and stable overexpression of CsMYB96 reduced water loss from both citrus fruit and Arabidopsis.

中文翻译：

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CsMYB96通过同时调节水分运输和蜡生物合成来赋予柑橘类水果抗失水性

Citrus sinensis R2R3 MYB 转录因子 (CsMYB96) 先前已被证明与果实中与蜡生物合成相关的许多基因的表达密切相关。在这项研究中，发现 CsMYB96 通过同时调节编码质膜内在蛋白 (CsPIP) 和蜡相关基因的基因的表达来减轻水分流失。表达谱表明CsPIP1;1和CsPIP2;4具有代表其他水通道蛋白的高表达，它们在采后柑橘果皮中表达下调。CsPIP2;4 被进一步表征为主要的 CsPIP，具有高表达和高水通道活性。CsPIP2;4 的瞬时过表达加速了柑橘类水果的水分流失。计算机分析进一步表明，CsMYB96 的表达与 CsPIPs 的表达呈显着负相关。体内和体外实验证实 CsMYB96 能够直接抑制 CsPIPs 的表达。此外，CsMYB96 能够激活蜡相关基因并促进蜡生物合成以防止水分流失。CsMYB96 的瞬时和稳定过表达减少了柑橘类水果和拟南芥的水分流失。