Pullulan is an important exopolysaccharide commercially obtained from ubiquitous fungi Aureobasidium pullulans. Pullulan has unique physicochemical properties, due to which it has wide applications in the food, pharmaceutical and biomedical industries. Various synthetic media and agro-industrial wastes have been utilized for the production of pullulan. Agro-industrial waste has become a preferred substrate for biotransformation due to its economical aspect and nutritional attributes. Pullulan is one of the valuable products produced by the biotransformation of agro-based wastes. Numerous factors which influence the pullulan production are the type of substrate, the addition of carbon source, nitrogen source, pH of the medium, aeration/ agitation speed and temperature. The cost, productivity of the product and efficiency depends on the selection of raw material, type of fermentation (bioprocess), type of strain and downstream processing (recovery). This review focuses on pullulan production from agro-based wastes, the influence of factors and its applications.

普鲁兰多糖是一种重要的胞外多糖，可从普遍存在的真菌Aureobasidium pullulans 中获得. 普鲁兰多糖具有独特的理化性质，因此在食品、制药和生物医学行业有着广泛的应用。各种合成介质和农业工业废物已被用于生产支链淀粉。由于其经济方面和营养属性，农业工业废物已成为生物转化的首选基质。普鲁兰多糖是通过农业废弃物生物转化生产的有价值的产品之一。影响支链淀粉生产的许多因素是底物的类型、碳源的添加、氮源、培养基的 pH 值、通气/搅拌速度和温度。产品的成本、生产力和效率取决于原材料的选择、发酵类型（生物过程）、菌株类型和下游加工（回收）。