Determination of the Intracellular Complexation of Inorganic and Methylmercury in Cyanobacterium Synechocystis sp. PCC 6803,Environmental Science & Technology

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Determination of the Intracellular Complexation of Inorganic and Methylmercury in Cyanobacterium Synechocystis sp. PCC 6803
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-09-30 , DOI: 10.1021/acs.est.1c01732
Javier Garcia-Calleja ₁ , Thibaut Cossart ₂ , Zoyne Pedrero ₁ , João P Santos ₂ , Laurent Ouerdane ₁ , Emmanuel Tessier ₁ , Vera I Slaveykova ₂ , David Amouroux ₁

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Understanding of mercury (Hg) complexation with low molecular weight (LMW) bioligands will help elucidate its speciation. In natural waters, the rate of this complexation is governed by physicochemical, geochemical, and biochemical parameters. However, the role of bioligands involved in Hg intracellular handling by aquatic microorganisms is not well documented. Here, we combine the use of isotopically labeled Hg species (inorganic and monomethylmercury, iHg and MeHg) with gas or liquid chromatography coupling to elemental and molecular mass spectrometry to explore the role of intracellular biogenic ligands involved in iHg and MeHg speciation in cyanobacterium Synechocystis sp. PCC 6803, a representative phytoplankton species. This approach allowed to track resulting metabolic and newly found intracellular Hg biocomplexes (e.g., organic thiols) in Synechocystis sp. PCC 6803 finding different intracellular Hg species binding affinities with both high and low molecular weight (HMW and LMW) bioligands in the exponential and stationary phase. Furthermore, the parallel detection with both elemental and molecular ionization sources allowed the sensitive detection and molecular identification of glutathione (GSH) as the main low molecular weight binding ligand to iHg ((GS)₂-Hg) and MeHg (GS-MeHg) in the cytosolic fraction. Such a novel experimental approach expands our knowledge on the role of biogenic ligands involved in iHg and MeHg intracellular handling in cyanobacteria.

中文翻译：

测定蓝藻集胞藻中无机和甲基汞的细胞内复合。PCC 6803

了解汞 (Hg) 与低分子量 (LMW) 生物配体的络合将有助于阐明其形态。在天然水中，这种络合的速率受物理化学、地球化学和生物化学参数的控制。然而，生物配体在水生微生物细胞内处理汞中的作用没有得到很好的记录。在这里，我们将同位素标记的 Hg 物种（无机和单甲基汞、iHg 和 MeHg）与气相或液相色谱与元素和分子质谱联用相结合，以探索参与 iHg 和 MeHg 物种形成的细胞内生物配体在蓝藻集胞藻中的作用sp. PCC 6803，一种代表性的浮游植物物种。这种方法允许跟踪在集胞藻中产生的代谢和新发现的细胞内汞生物复合物（例如，有机硫醇）。PCC 6803 在指数相和固定相中发现不同的细胞内 Hg 物种与高和低分子量（HMW 和 LMW）生物配体的结合亲和力。此外，使用元素和分子电离源的平行检测允许对作为 iHg 的主要低分子量结合配体的谷胱甘肽 (GSH) 进行灵敏检测和分子鉴定 ((GS) ₂-Hg) 和 MeHg (GS-MeHg) 在细胞质部分。这种新颖的实验方法扩展了我们对参与蓝藻中 iHg 和 MeHg 细胞内处理的生物配体的作用的认识。

更新日期：2021-10-19

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