This study compared growth performance between female and male transgenic channel catfish, Ictalurus punctatus, containing channel catfish growth hormone full-length cDNA driven by the ocean pout antifreeze protein promoter, opAFP-ccGH, the rainbow trout metallothionein promoter, rtMT-ccGH, or both constructs, and their non-transgenic siblings in earthen ponds at 16 and 48 months of age. Body weight between the transgenic and their non-transgenic siblings differed (P < 0.001) at all ages. Transgenic F₂ opAFP-ccGH grew 1.51- to 2.58-, F₂ rtMT-ccGH grew 1.44- to 2.99- and F₁fish transgenic for both constructs grew 1.36- to 2.92- fold larger than their non-transgenic sibling controls, depending upon age and sex. Body weight of the transgenic GH males was significantly higher than those of the transgenic GH females at 16 months of age (P < 0.001). However, body weight of the transgenic GH females was significantly higher (P < 0.001) compared with those of the transgenic GH males at 48 months of age, but not for the double transgenics (P > 0.05). In the case of non-transgenic GH siblings, males were larger than females at both 16 and 48 months of age (P < 0.001). Sexually dimorphic responses to GH transgenes were the opposite after sexual maturation. When critically low dissolved oxygen levels were encountered, survival of transgenic male and female opAFP-ccGH channel catfish was lower than that of controls (P = 0.004), as well as rtMT-ccGH females (P = 0.11), which is not surprising since the largest fish are most likely to succumb during an oxygen depletion.

本研究比较了雌性和雄性转基因鲶鱼 ( Ictalurus punctatus ) 之间的生长性能，该鲶鱼含有由洋鲶抗冻蛋白启动子、opAFP-ccGH、虹鳟金属硫蛋白启动子、rtMT-ccGH 或两者驱动的鲶鱼生长激素全长 cDNA构建体，以及它们在 16 个月和 48 个月大的土池中的非转基因兄弟姐妹。转基因和非转基因同胞之间的体重 在所有年龄段都有差异（ P < 0.001）。转基因 F ₂ opAFP-ccGH 增长 1.51- 至 2.58-，F ₂ rtMT-ccGH 增长 1.44- 至 2.99- 和 F ₁根据年龄和性别，两种构建体的转基因鱼比它们的非转基因同胞对照增长了 1.36 到 2.92 倍。16个月大时，转基因GH雄性的体重显着高于转基因GH雌性（P  < 0.001）。然而，在 48 个月大时，转基因 GH 雌性的体重显着高于转基因 GH 雄性的体重（P  <0.001），但双转基因不显着（P  > 0.05）。在非转基因 GH 同胞的情况下，男性在 16 个月和 48 个月大时均大于女性（P < 0.001)。在性成熟后，对 GH 转基因的性二态反应则相反。当遇到极低的溶解氧水平时，转基因雄性和雌性 opAFP-ccGH 鲶鱼的存活率低于对照（P  = 0.004）以及 rtMT-ccGH 雌性（P  = 0.11），这并不奇怪，因为最大的鱼最有可能在氧气耗尽时死亡。