Background Human epidermal stem cells (hESCs) play an important role in re-epithelialization and thereby in facilitating wound healing, while an effective way to activate hESCs remains to be explored. Calcium silicate (CS) is a form of bioceramic that can alter cell behavior and promote tissue regeneration. Here, we have observed the effect of CS on hESCs and investigated its possible mechanism. Methods Using a mouse full-thickness skin excision model, we explored the therapeutic effect of CS on wound healing and re-epithelialization. In vitro, hESCs were cultured with diluted CS ion extracts (CSIEs), and the proliferation, migration ability and stemness of hESCs were evaluated. The effects of CS on the epidermal growth factor (EGF), epidermal growth factor receptor (EGFR) and extracellular signal-related kinase (ERK) signaling pathway were also explored. Results In vivo, CS accelerated wound healing and re-epithelialization. Immunohistochemistry demonstrated that CS upregulated cytokeratin 19 and integrin β1 expression, indicating that CS improved hESCs stemness. In vitro studies confirmed that CS improved the biological function of hESCs. And the possible mechanism could be due to the activation of the EGF/EGFR/ERK signaling pathway. Conclusion CS can promote re-epithelialization and improve the biological functions of hESCs via activating the EGF/EGFR/ERK signaling pathway.

中文翻译：

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硅酸钙通过 EGF/EGFR/ERK 介导的表皮干细胞功能促进，增强上皮细胞再生，加速皮肤伤口愈合


背景人表皮干细胞（hESC）在上皮再形成中发挥着重要作用，从而促进伤口愈合，而激活hESC的有效方法仍有待探索。硅酸钙（CS）是一种生物陶瓷，可以改变细胞行为并促进组织再生。在这里，我们观察了CS对hESC的影响并研究了其可能的机制。方法利用小鼠全层皮肤切除模型，探讨CS对伤口愈合和上皮再形成的治疗作用。在体外，用稀释的CS离子提取物（CSIEs）培养hESCs，并评估hESCs的增殖、迁移能力和干性。还探讨了CS对表皮生长因子（EGF）、表皮生长因子受体（EGFR）和细胞外信号相关激酶（ERK）信号通路的影响。结果 在体内，CS 加速了伤口愈合和上皮化。免疫组织化学表明，CS 上调细胞角蛋白 19 和整合素 β1 的表达，表明 CS 改善了 hESC 的干性。体外研究证实CS改善了hESC的生物学功能。其可能的机制可能是由于EGF/EGFR/ERK信号通路的激活。结论 CS可通过激活EGF/EGFR/ERK信号通路促进hESCs再上皮化，改善其生物学功能。