Although strawberry can be cultivated in all types of climate, it grows best in temperate climates, limiting its cultivation in certain countries or regions with tropical or subtropical climates. The objective of this study was to verify the genetic diversity of 34 pre-selected day-neutral strawberry genotypes in humid tropical and subtropical conditions through characterization of morpho-agronomic characteristics and molecular markers. The selection was based on qualitative and quantitative morpho-agronomic traits, using the Gower algorithm. Molecular characterization was performed with AFLP markers, using four combinations of selective primers. Six representative variables were subjected to principal component analysis (PCA) and the Mulamba and Mock selection index. Soluble solids and luminosity of the outer part of the fruit did not differ among genotypes but presented values ​​above those usually found. AFLP markers detected 80% polymorphism, confirming high genetic variability among genotypes. Clustering based on molecular and agronomic data separated the genotypes into three groups, and the Mulamba and Mock index did not select individuals derived from 'Albion' or RVFS06. PCA highlighted the genotypes RVCA16M-1, RVDA11M-3, RVDA11M-4, and RVDA11M-25 as superior for most of the evaluated traits compared with the commercial cultivars 'Albion' and 'Monterey'. The PCA and selection index results indicate the reliability of the multivariate analysis for selecting superior strawberry genotypes for cultivation in tropical regions. Of the 34 genotypes analyzed, four stood out with potential for immediate cultivation.

草莓虽然可以在所有类型的气候下种植，但它在温带气候下生长最好，限制了它在某些热带或亚热带气候的国家或地区的种植。本研究的目的是通过形态农学特征和分子标记的表征，验证 34 个预选的日间中性草莓基因型在潮湿的热带和亚热带条件下的遗传多样性。选择基于定性和定量的形态农艺性状，使用 Gower 算法。使用 AFLP 标记物进行分子表征，使用四种选择性引物组合。六个代表性变量进行主成分分析 (PCA) 和 Mulamba 和模拟选择指数。水果外部的可溶性固体和光度在基因型之间没有差异，但呈现的值高于通常发现的值。AFLP 标记检测到 80% 的多态性，证实了基因型之间的高遗传变异性。基于分子和农艺数据的聚类将基因型分为三组，Mulamba 和 Mock 指数没有选择来自“Albion”或 RVFS06 的个体。PCA 强调基因型 RVCA16M-1、RVDA11M-3、RVDA11M-4 和 RVDA11M-25 与商业栽培品种 'Albion' 和 'Monterey' 相比，在大多数评估性状方面具有优越性。PCA和选择指数结果表明多变量分析在选择用于热带地区栽培的优良草莓基因型方面的可靠性。在分析的 34 种基因型中，