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Evaluation of ORAC, IR and NMR metabolomics for predicting ripening stage and variety in melon (Cucumis melo L.)
Food Chemistry ( IF 8.5 ) Pub Date : 2021-09-29 , DOI: 10.1016/j.foodchem.2021.131263
Ana Isabel Tristán 1 , Ana Cristina Abreu 1 , Luis Manuel Aguilera-Sáez 1 , Araceli Peña 2 , Andrés Conesa-Bueno 3 , Ignacio Fernández 1
Affiliation  

A combination of FTIR- and NMR-based metabolomics approaches coupled to multivariate data analysis techniques was used for the first time to characterize and explore the metabolic changes along the ripening of two Spanish melon landraces (Jimbee N and Jimbee XL). Furthermore, the fatty acids profile and the antioxidant capacity were investigated by GC-FID and ORAC method, respectively. Despite FTIR was able to identify changes in the sugars content between fruits of different maturity stages, it failed to discriminate between both varieties, being exceeded by NMR in both situations. Increased fruit maturation led to an increase of sucrose, fatty acids and β-carotene, accompanied by a decrease of glucose, fructose, citrate, amino acids, and polyphenols (which were positively correlated with fruits antioxidant activity).



中文翻译:

评估 ORAC、IR 和 NMR 代谢组学以预测甜瓜 (Cucumis melo L.) 的成熟阶段和品种

基于 FTIR 和 NMR 的代谢组学方法与多变量数据分析技术相结合,首次用于表征和探索两种西班牙甜瓜地方品种(Jimbee N 和 Jimbee XL)成熟过程中的代谢变化。此外,分别通过 GC-FID 和 ORAC 方法研究了脂肪酸谱和抗氧化能力。尽管 FTIR 能够识别不同成熟阶段水果之间糖含量的变化,但它无法区分这两个品种,在两种情况下都被 NMR 超过。果实成熟度增加导致蔗糖、脂肪酸和β的增加-胡萝卜素,伴随着葡萄糖、果糖、柠檬酸盐、氨基酸和多酚(与水果抗氧化活性呈正相关)的减少。

更新日期:2021-10-17
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