Mass Spectrometry of Nucleic Acid Noncovalent Complexes,Chemical Reviews

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Mass Spectrometry of Nucleic Acid Noncovalent Complexes
Chemical Reviews ( IF 51.4 ) Pub Date : 2021-09-30 , DOI: 10.1021/acs.chemrev.1c00386
Eric Largy ₁ , Alexander König ₁ , Anirban Ghosh ₁ , Debasmita Ghosh ₁ , Sanae Benabou ₁ , Frédéric Rosu ₂ , Valérie Gabelica ₁

Affiliation

Nucleic acids have been among the first targets for antitumor drugs and antibiotics. With the unveiling of new biological roles in regulation of gene expression, specific DNA and RNA structures have become very attractive targets, especially when the corresponding proteins are undruggable. Biophysical assays to assess target structure as well as ligand binding stoichiometry, affinity, specificity, and binding modes are part of the drug development process. Mass spectrometry offers unique advantages as a biophysical method owing to its ability to distinguish each stoichiometry present in a mixture. In addition, advanced mass spectrometry approaches (reactive probing, fragmentation techniques, ion mobility spectrometry, ion spectroscopy) provide more detailed information on the complexes. Here, we review the fundamentals of mass spectrometry and all its particularities when studying noncovalent nucleic acid structures, and then review what has been learned thanks to mass spectrometry on nucleic acid structures, self-assemblies (e.g., duplexes or G-quadruplexes), and their complexes with ligands.

中文翻译：

核酸非共价配合物的质谱分析

核酸一直是抗肿瘤药物和抗生素的首要目标之一。随着在基因表达调控中新的生物学作用的揭示，特定的 DNA 和 RNA 结构已成为非常有吸引力的目标，特别是当相应的蛋白质不可成药时。用于评估靶标结构以及配体结合化学计量、亲和力、特异性和结合模式的生物物理测定是药物开发过程的一部分。质谱法作为一种生物物理方法具有独特的优势，因为它能够区分混合物中存在的每种化学计量。此外，先进的质谱方法（反应探测、碎裂技术、离子迁移谱、离子光谱）提供了有关配合物的更详细信息。这里，

更新日期：2021-09-30

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