Haploids have often been used to simplify the genetic complexity of potato due to its high heterozygosity and autotetraploidy. We maintained some haploid plants of wild potato species in vitro that were produced through anther culture almost a half century ago. Among them, a diploid Solanum bulbocastanum and its monohaploid, three monohaploids of S. verrucosum, a disomic tetraploid S. stoloniferum and its dihaploid (all Mexican species) and a dihaploid of the disomic tetraploid S. acaule (South American species) were analyzed morphologically, cytologically and by molecular markers. Their species identity and ploidy levels were confirmed to be correct except for those of a monoploid S. bulbocastanum, which had already experienced natural chromosome doubling. The monohaploids and chromosome-doubled monohaploid were all homozygous in 19,889 SNP loci surveyed. Two of three monohaploids of S. verrucosum were morphologically different but similar by SNP analysis. Most of the heterozygous loci of the tetraploid S. stoloniferum were in a duplex condition. The heterozygous loci of the dihaploid S. stoloniferum were mostly duplexed in the tetraploid S. stoloniferum, suggesting that the alleles are fixed in homoeologous loci. Therefore, each of these haploid-plant genomes is homozygous and is suitable for whole-genome sequencing.

由于其高杂合性和同源四倍体，单倍体经常被用来简化马铃薯的遗传复杂性。我们在体外维持了一些野生马铃薯物种的单倍体植物，这些植物是近半个世纪前通过花药培养产生的。其中，对1个二倍体Solanumbulbocastanum及其单单倍体、3个S. verrucosum单单倍体、1个二体四倍体S. stoloniferum及其二单倍体（均为墨西哥种）和1个二体四倍体S. acaule（南美种）的二倍体进行形态分析。 ，细胞学和分子标记。除了单倍体S.bulbocastanum之外，它们的物种身份和倍性水平被证实是正确的，已经经历了自然染色体加倍。在调查的19,889个SNP位点中，单单倍体和染色体加倍的单单倍体均为纯合子。S. verrucosum的三个单单倍体中的两个在形态上不同但通过 SNP 分析相似。四倍体S. stoloniferum 的大多数杂合基因座处于双链状态。在双单倍体的杂合基因座S.三棱大多双工在四倍体S.三棱，这表明等位基因被固定在部分同源基因座。因此，这些单倍体植物基因组中的每一个都是纯合的，适用于全基因组测序。