A novel fast-response and highly selective AIEgen fluorescent probe for visualizing peroxynitrite in living cells, C. elegans and inflammatory mice,Analyst

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A novel fast-response and highly selective AIEgen fluorescent probe for visualizing peroxynitrite in living cells, C. elegans and inflammatory mice
Analyst ( IF 3.6 ) Pub Date : 2021-09-08 , DOI: 10.1039/d1an01374b
Ya-Xi Ye ₁ , Xin-Yue Chen ₁ , Ya-Wen Yu ₁ , Qing Zhang ₁ , Xiao-Wen Wei ₁ , Zhong-Chang Wang ₁ , Bao-Zhong Wang ₁ , Qing-Cai Jiao ₁ , Hai-Liang Zhu ₁

Affiliation

Most of the ONOO⁻ fluorescent probes have restricted applications because of their aggregation-caused quenching (ACQ) effect, long response time and low fluorescence enhancement. Herein, we developed a novel AIEgen fluorescent probe (PE-XY) based on a benzothiazole and quinolin scaffold with high sensitivity and selectivity for imaging of ONOO⁻. The results indicated that probe PE-XY exhibited fast response towards ONOO⁻ with 2000-fold enhancement of fluorescence intensity ratio in vitro. Moreover, PE-XY exhibited a relatively high sensitivity (limit of detection: 8.58 nM), rapid response (<50 s), high fluorescence quantum yield (δ = 0.81) and excellent selectivity over other analytes towards ONOO⁻ in vitro. Furthermore, PE-XY was successfully applied to detect endogenous ONOO⁻ levels in living HeLa cells, C. elegans and inflammatory mice with low cytotoxicity. Overall, this work provided a novel fast-response and highly selective AIEgen fluorescent probe for real-time monitoring ONOO⁻ fluctuations in living systems.

中文翻译：

一种新型快速响应和高选择性 AIEgen 荧光探针，用于可视化活细胞、秀丽隐杆线虫和炎症小鼠中的过氧亚硝酸盐

大多数 ONOO ^-荧光探针的应用受到限制，因为它们具有聚集引起的猝灭 (ACQ) 效应、响应时间长和荧光增强低。在此，我们开发了一种基于苯并噻唑和喹啉支架的新型 AIEgen 荧光探针 ( PE-XY )，对 ONOO ^{- 的}成像具有高灵敏度和选择性。结果表明，探针PE-XY表现出快速响应朝ONOO ^-与的荧光强度比2000倍的增强体外。此外，PE-XY表现出相对较高的灵敏度（检测限：8.58 nM）、快速响应（<50 s）、高荧光量子产率（δ= 0.81) 并且在体外对 ONOO ^{- 的} 选择性优于其他分析物。此外，PE-XY已成功应用于检测活 HeLa 细胞、秀丽隐杆线虫和具有低细胞毒性的炎症小鼠中的内源性 ONOO ^-水平。总体而言，这项工作提供了一个新的快速响应和高选择性AIEgen荧光探针用于实时监控ONOO ^-在生命系统的波动。

更新日期：2021-09-29

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