A double-tetrahedral DNA framework based electrochemical biosensor for ultrasensitive detection and release of circulating tumor cells,Analyst

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A double-tetrahedral DNA framework based electrochemical biosensor for ultrasensitive detection and release of circulating tumor cells
Analyst ( IF 3.6 ) Pub Date : 2021-09-08 , DOI: 10.1039/d1an01470f
Chenguang Wang _{1,

2} , Yi Xu ₁ , Xiaoshuang Zhao ₃ , Shuainai Li _{1,

2} , Qiuling Qian _{1,

2} , Wei Wang ₄ , Xianqiang Mi _{1,

2,

3,

5,

6}

Affiliation

Detecting circulating tumor cells (CTCs) in patients’ blood is essential for early diagnosis, precise treatment and prognosis of cancer. Yet due to CTCs being extremely rare in the peripheral blood of patients, it is still a challenge to detect CTCs with high sensitivity and high selectivity. Here, we developed a double-tetrahedral DNA framework (DTDF) based electrochemical biosensor system (E-CTC sensor system) for ultrasensitive detection and release of CTCs. In this work, an upright tetrahedral DNA framework (UTDF) was used as a rigid scaffold to modify a screen-printed gold electrode (SPGE), and an inverted tetrahedral DNA framework (ITDF) provided three vertex chains to multivalently bind with aptamers. Meanwhile, a streptavidin tagged horseradish peroxidase homopolymer (SA-polyHRP) was linked to biotin-modified aptamers to significantly amplify the signal. Moreover, the captured CTCs could be effectively released via benzonase nuclease with little cell damage. Our E-CTC sensor system achieved a linear range from 1 to 10⁵ MCF-7 cells with an ultralow detection limit of 1 cell. The release efficiency reached 88.1%–97.6% and the viability of the released cells reached up to 98%. We also detected the MCF-7 cells in mimic whole blood samples, suggesting that the E-CTC sensor system shows promise for use in clinical research.

中文翻译：

基于双四面体 DNA 框架的电化学生物传感器用于循环肿瘤细胞的超灵敏检测和释放

检测患者血液中的循环肿瘤细胞 (CTC) 对于癌症的早期诊断、精确治疗和预后至关重要。然而，由于CTCs在患者外周血中极为罕见，高灵敏度和高选择性检测CTCs仍然是一个挑战。在这里，我们开发了一种基于双四面体 DNA 框架（DTDF）的电化学生物传感器系统（E-CTC 传感器系统），用于 CTC 的超灵敏检测和释放。在这项工作中，直立四面体 DNA 框架 (UTDF) 用作刚性支架来修饰丝网印刷金电极 (SPGE)，倒置四面体 DNA 框架 (ITDF) 提供三个顶点链与适体多价结合。同时，链霉亲和素标记的辣根过氧化物酶均聚物 (SA-polyHRP) 与生物素修饰的适体连接以显着放大信号。此外，捕获的 CTC 可以有效释放通过benzonase 核酸酶，对细胞损伤很小。我们的 E-CTC 传感器系统实现了从 1 到 10 ⁵ MCF-7 细胞的线性范围，具有 1细胞的超低检测限。释放效率达到88.1%~97.6%，释放的细胞活力达到98%。我们还在模拟全血样本中检测到 MCF-7 细胞，这表明 E-CTC 传感器系统有望用于临床研究。

更新日期：2021-09-29

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