Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell,Analyst

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Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell
Analyst ( IF 3.6 ) Pub Date : 2021-09-08 , DOI: 10.1039/d1an01506k
Jingyu Xiao ₁ , Meng Tian ₁ , Lei Su ₂ , Yu Bao ₃ , Li Niu ₃ , Xueji Zhang ₂

Affiliation

Endogenous H₂O₂ generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform. With PMA as the stimulus, the cell released a small amount of H₂O₂ and its mitochondrial membrane potential (MMP) decrease was smaller, compared with that on the PDMS substrates.

中文翻译：

检测聚多巴胺 (PDA) 涂层的聚二甲基硅氧烷 (PDMS) 基材对单个 HeLa 细胞释放 H2O2 的影响

使用自制的光电双检测平台检测由单个 HeLa 细胞产生的内源性 H ₂ O ₂，该细胞在 25 mM 葡萄糖培养条件下粘附在 PDA 涂层的 PDMS 基质上。与PDMS底物相比，以PMA为刺激，细胞释放少量H ₂ O ₂，其线粒体膜电位（MMP）降低幅度较小。

更新日期：2021-09-29

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