The artificial light used in growth chambers is usually devoid of green (G) light, which is considered to be less photosynthetically efficient than blue (B) or red (R) light. To verify the role of G light supplementation in the spectrum, we modified the RB spectrum by progressively replacing R light with an equal amount of G light. The tomato plants were cultivated under 100 µmol m^–2 s^–1 of five different combinations of R (35–75%) and G light (0–40%) in the presence of a fixed proportion of B light (25%) provided by light-emitting diodes (LEDs). Substituting G light for R altered the plant’s morphology and partitioning of biomass. We observed a decrease in the dry biomass of leaves, which was associated with increased biomass accumulation and the length of the roots. Moreover, plants previously grown under the RGB spectrum more efficiently utilized the B light that was applied to assess the effective quantum yield of photosystem II, as well as the G light when estimated with CO₂ fixation using RB + G light-response curves. At the same time, the inclusion of G light in the growth spectrum reduced stomatal conductance (g_s), transpiration (E) and altered stomatal traits, thus improving water-use efficiency. Besides this, the increasing contribution of G light in place of R light in the growth spectrum resulted in the progressive accumulation of phytochrome interacting factor 5, along with a lowered level of chalcone synthase and anthocyanins. However, the plants grown at 40% G light exhibited a decreased net photosynthetic rate (P_n), and consequently, a reduced dry biomass accumulation, accompanied by morphological and molecular traits related to shade-avoidance syndrome.

生长室中使用的人造光通常没有绿 (G) 光，绿 (G) 光被认为比蓝光 (B) 或红 (R) 光的光合作用效率低。为了验证 G 光补充在光谱中的作用，我们通过用等量的 G 光逐渐替换 R 光来修改 RB 光谱。番茄植株在 100 µmol m ^–2  s ^–1在发光二极管 (LED) 提供固定比例的 B 光 (25%) 的情况下，R (35–75%) 和 G 光 (0–40%) 的五种不同组合。用 G 光代替 R 改变了植物的形态和生物量的分配。我们观察到叶子的干生物量减少，这与生物量积累和根的长度增加有关。此外，先前在 RGB 光谱下生长的植物更有效地利用了用于评估光系统 II 的有效量子产率的 B 光，以及使用 RB + G 光响应曲线通过 CO _{2固定估计时的 G 光。}同时，在生长光谱中加入 G 光会降低气孔导度 ( g _s )、蒸腾作用 ( E) 和改变气孔性状，从而提高水分利用效率。除此之外，G 光代替 R 光在生长光谱中的贡献增加导致光敏色素相互作用因子 5 的逐渐积累，同时查尔酮合酶和花青素水平降低。然而，在 40% G 光下生长的植物表现出净光合速率 ( Pn ) 降低，因此干生物量积累减少，并伴有与避荫综合征相关的形态和分子特征_。