A vesicle-enhanced liquid-phase pulsed discharge (V-LPD) was proposed to extract polyphenols from green tea leaves. The optimal extraction conditions obtained by the process optimization were 3% (w/v) dodecyl trimethy lammonium bromide (DTAB)/sodium dodecyl sulfate (SDS) vesicle, 10% (v/v) ethanol/vesicle, 66 mL/g liquid-to-solid ratio, 5 kV discharge voltage, and 2.5 min extraction time. Under these optimal conditions, the polyphenols yield was 124.650 ± 1.197 mg/g, which was observably higher than that by heat reflux extraction (HRE) (108.156 ± 1.437 mg/g) for 45 min and that by ethanol-enhanced LPD (116.123 ± 1.164 mg/g) for 4 min. The energy consumptions of V-LDP and ethanol-enhanced LPD were far lower than that by HRE. Similar chemical constituents were observed by HPLC analysis in three extraction methods, and the extracts obtained by V-LPD had the highest antioxidant capacity. Therefore, the V-LPD is a time-saving and energy-saving method for the extraction of polyphenols.

Industrial relevance

Green tea is considered to be an important source of polyphenols. The potential of V-LPD has been demonstrated as a time-saving and energy-saving alternative using vesicle solvents for the simultaneous cell disruption and strengthening diffusion of polyphenols from green tea leaves. The polyphenols obtained with V-LPD process can be used in an array of pharmaceutical, cosmetic and food applications.

中文翻译：

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囊泡增强液相脉冲放电提取绿茶叶中的多酚

提出了一种囊泡增强液相脉冲放电 (V-LPD) 从绿茶叶中提取多酚。工艺优化得到的最佳提取条件为3%（w / v）十二烷基三甲基溴化铵（DTAB）/十二烷基硫酸钠（SDS）囊泡，10%（v/v) 乙醇/囊泡，66 mL/g 液固比，5 kV 放电电压和 2.5 分钟提取时间。在这些最佳条件下，多酚的产率为 124.650 ± 1.197 mg/g，明显高于热回流提取 (HRE) 45 min 的产率 (108.156 ± 1.437 mg/g) 和乙醇增强 LPD 的产率 (116.123 ± 1.164 毫克/克）持续 4 分钟。V-LDP 和乙醇增强 LPD 的能耗远低于 HRE。三种提取方法的HPLC分析均观察到相似的化学成分，其中V-LPD提取物的抗氧化能力最高。因此，V-LPD是一种省时、节能的多酚提取方法。

工业相关性

绿茶被认为是多酚的重要来源。V-LPD 的潜力已被证明是一种节省时间和节能的替代品，它使用囊泡溶剂同时破坏细胞并加强绿茶叶中多酚的扩散。通过 V-LPD 工艺获得的多酚可用于一系列制药、化妆品和食品应用。