Chordomas are low-grade malignancies accounting for 1–4% of primary bone malignancies. Moreover, local recurrences increase the rate of metastasis. Our previous study identified the far upstream element (FUSE)-binding protein 1 (FUBP1) as a biomarker and potential therapeutic target for chordoma. In this study, lncRNA KRT8P41 was identified as a lncRNA positively correlated with FUBP1. In chordoma patients, higher lncRNA KRT8P41 expression was correlated with a poorer prognosis. LncRNA KRT8P41 silencing significantly inhibited chordoma cell proliferation and invasion. miR-193a was negatively correlated with lncRNA KRT8P41 and FUBP1; lncRNA KRT8P41 inhibited miR-193a expression, and miR-193a inhibited FUBP1 expression. Furthermore, miR-193a directly bound to lncRNA KRT8P41 and FUBP1 and lncRNA KRT8P41 competed with FUBP1 for miR-193a binding and relieved miR-193a-mediated FUBP1 inhibition. LncRNA KRT8P41 silencing inhibited, whereas miR-193a inhibition promoted chordoma cell proliferation and invasion; the inhibition of miR-193a attenuated the roles of lncRNA KRT8P41. Within chordoma tissues, the expression of miR-193a was decreased, and the expression of FUBP1 increased compared to normal control tissues. LncRNA KRT8P41 exhibited a positive correlation with FUBP1 and a negative correlation with miR-193a in vivo. Therefore, it was concluded that lncRNA KRT8P41, miR-193a-3p, and FUBP1 form a lncRNA-miRNA-mRNA axis, modulating the proliferation and invasion of chordoma cells.

脊索瘤是低度恶性肿瘤，占原发性骨恶性肿瘤的 1-4%。此外，局部复发会增加转移率。我们之前的研究将远上游元件 (FUSE) 结合蛋白 1 (FUBP1) 确定为脊索瘤的生物标志物和潜在治疗靶点。在本研究中，lncRNA KRT8P41 被鉴定为与 FUBP1 正相关的 lncRNA。在脊索瘤患者中，较高的 lncRNA KRT8P41 表达与较差的预后相关。LncRNA KRT8P41 沉默显着抑制脊索瘤细胞增殖和侵袭。miR-193a与lncRNA KRT8P41和FUBP1呈负相关；lncRNA KRT8P41抑制miR-193a表达，miR-193a抑制FUBP1表达。此外，miR-193a 直接与 lncRNA KRT8P41 和 FUBP1 结合，lncRNA KRT8P41 与 FUBP1 竞争 miR-193a 结合并解除 miR-193a 介导的 FUBP1 抑制。LncRNA KRT8P41 沉默抑制，而 miR-193a 抑制促进脊索瘤细胞增殖和侵袭；miR-193a 的抑制减弱了 lncRNA KRT8P41 的作用。在脊索瘤组织中，与正常对照组织相比，miR-193a 的表达降低，FUBP1 的表达增加。LncRNA KRT8P41与FUBP1呈正相关，与miR-193a呈负相关 与正常对照组织相比，miR-193a的表达降低，FUBP1的表达增加。LncRNA KRT8P41与FUBP1呈正相关，与miR-193a呈负相关 与正常对照组织相比，miR-193a的表达降低，FUBP1的表达增加。LncRNA KRT8P41与FUBP1呈正相关，与miR-193a呈负相关体内。因此，推断lncRNA KRT8P41、miR-193a-3p和FUBP1形成lncRNA-miRNA-mRNA轴，调节脊索瘤细胞的增殖和侵袭。