The genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified W. confusa as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain W. confusa LM1, which is the first genome of an insect-derived W. confusa strain with one complete chromosome and one complete plasmid. Among all W. confusa strains, W. confusa LM1 had the largest genome. Its genome was the closest to that of W. confusa 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known Weissella species and W. confusa strains. Based on genomic analysis, the pan-genome of W. confusa is in an open state. Most strains of W. confusa had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that W. confusa strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of W. confusa LM1 in locust biology and their potential application to locust management.

属 魏塞拉由于其多种功能和益生菌潜力，正引起越来越多的关注。特别是，该物种威瑟菌已知在工业应用中具有巨大潜力，并表现出许多生物学功能。然而，关于昆虫中这种细菌的知识没有被调查。在这里，我们隔离并确定了W. confusa作为迁徙蝗虫肠道中的主要乳酸菌。我们将此菌株命名为W. confusa LM1，这是昆虫衍生的第一个基因组 W. confusa具有一条完整染色体和一个完整质粒的菌株。全部当中W. confusa 菌株， W. confusaLM1 拥有最大的基因组。它的基因组最接近于W. confusa1001271B_151109_G12，一种来自人类粪便的菌株。我们的结果提供了已知的准确进化关系魏塞拉 物种和 W. confusa菌株。基于基因组分析，泛基因组W. confusa处于开放状态。大多数菌株W. confusa具有独特的基因，表明这些菌株可以适应不同的生态位和生物。然而，菌株特异性基因的变异确实代表了与其宿主和生态位的显着相关性。预计这些菌株产生次级代谢物的潜力较低。此外，未鉴定出抗生素抗性基因。同时未发现与毒素产生和分泌系统相关的毒力因子，说明W. confusa菌株不足以产生毒力。我们的研究有助于发现W. confusa 蝗虫生物学中的 LM1 及其在蝗虫管理中的潜在应用。