It is found effective for phytoremediation of the guest soil spraying method by adding microbes to promote the growth of arbor leguminous plant on a high and steep rock slope. However, its underlying mechanisms remain elusive. Here, some experiments were conducted to explore the multifunctions of Penicillium simplicissimum NL-Z1 on rock weathering, nodule growth, and beneficial microbial regulation. The results show that P. simplicissimum NL-Z1 significantly increased the release of phosphorus, potassium, calcium, and magnesium from the rock by 226, 29, 24, and 95%, respectively, compared with that of the control. A significant increase of 153% in Indigofera pseudotinctoria Matsum nodule biomass, accompanied by an increase of 37% in the leguminous plant biomass was observed in the P. simplicissimum NL-Z1 treatment than in the control treatment. Interestingly, even though P. simplicissimum NL-Z1 itself became a minor microbial community in the soil, it induced a significant increase in Mortierella, which, as a beneficial microbe, can promote phosphate-solubilizing and plant growth. The results suggest that P. simplicissimum NL-Z1 could induce an imposed effect to promote leguminous plant growth, which may be conducive to the development of the phytoremediation technique for high and steep rock slope. The study provides a novel thought of using the indirect effect of microbes, i.e., promoting other beneficial microbes, to improve soil environment.

通过添加微生物促进高陡岩坡上乔木豆科植物的生长，对客土喷洒法进行植物修复是有效的。然而，其潜在机制仍然难以捉摸。在这里，进行了一些实验来探索简单青霉NL-Z1 对岩石风化、结核生长和有益微生物调节的影响。结果表明P. simplicissimum与对照相比，NL-Z1 显着增加了岩石中磷、钾、钙和镁的释放量，分别增加了 226%、29%、24% 和 95%。显着增加了 153%蓝靛蓝 根瘤生物量，伴随豆科植物生物量增加 37% P. simplicissimumNL-Z1处理优于对照处理。有趣的是，即使P. simplicissimum NL-Z1 本身成为土壤中的一个次要微生物群落，它诱导了显着增加 被孢霉属，作为一种有益微生物，可以促进磷酸盐溶解和植物生长。结果表明P. simplicissimumNL-Z1可以诱导豆科植物生长的强加效应，这可能有利于高陡岩坡植物修复技术的发展。该研究为利用微生物的间接作用，即促进其他有益微生物的生长，改善土壤环境提供了一种新思路。