Development of 105 SNP markers in endangered turtle species Pelodiscus sinensis using RAD-seq,Conservation Genetics Resources

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Development of 105 SNP markers in endangered turtle species Pelodiscus sinensis using RAD-seq
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2021-09-25 , DOI: 10.1007/s12686-021-01238-z
Qiming Xie ₁ , Fan Liu ₁ , Jilong Zhang ₁ , Xilei Li ₁ , Tiantian Chen ₁ , Shiping Su ₁ , Guoxia Fang ₂ , Rensheng Ma ₂

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Restriction site-associated DNA sequencing (RAD-seq) was used to develop single nucleotide polymorphisms (SNP) marker for the Pelodiscus sinensis, which is a commercially important species in China. A total of 105 high-quality SNP markers were identified. The genetic statistics showed that the minor allele frequency varied from 0.0571 to 0.3382 and the observed heterozygosity and the expected heterozygosity ranged from 0.1143 to 0.6765 and 0.1078 to 0.4477, respectively. These novel SNP markers will serve as a valuable genetic tool for future conservation and population genetics studies for P. sinensis.

更新日期：2021-09-28

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