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Enterobacteriaceae and Salmonella contamination of wild boar (Sus scrofa) carcasses: comparison between different sampling strategies
European Journal of Wildlife Research ( IF 1.8 ) Pub Date : 2021-09-25 , DOI: 10.1007/s10344-021-01531-0
Silvia Bonardi 1 , Cesare Tansini 1 , Antonio Cacchioli 1 , Laura Soliani 2 , Luca Poli 1 , Luca Lamperti 1 , Margherita Corradi 3 , Stefano Gilioli 3
Affiliation  

During 2020, a total of 64 wild boar carcasses were tested for Enterobacteriaceae count (EBC), Salmonella and Yersinia enterocolitica in the abdominal region (i) within 5 h after hunting in the game collection point and (ii) before dressing and processing in the game-handling establishment (GHE) (49 carcasses—average time interval between (i) and (ii): 4.3 days). Because of COVID-19 restrictions, 15 carcasses were transported to a near slaughterhouse (average time interval between (i) and (ii): 2.3 days). Mesenteric lymph nodes (MLNs) were collected and tested for Salmonella and Y. enterocolitica. Results are shown in relation to sampling A (49 carcasses—GHE) and sampling B (15 carcasses—slaughterhouse). Sampling A: EBC median values were (i) 2.51 log10 CFU/cm2 and (ii) 2.79 log10 CFU/cm2. EBC increase between (i) and (ii) was statistically significant (p = 0.001). Salmonella prevalence on carcasses varied from (i) 2.0 to (ii) 6.1%. Sampling B: EBC median values were (i) 3.1 log10 CFU/cm2 and (ii) 3.32 log10 CFU/cm2. EBC increase between (i) and (ii) was not statistically significant (p = 0.191). Salmonella prevalence on carcasses varied from (i) 6.7 to (ii) 0.0%. The prevalence (sampling A + B) of lymphatic Salmonella carriers was 7.8% (5/64). From carcasses and/or MNLs, the serovars Enteritidis, Typhimurium, Agama, Zaiman and Diarizonae O:50 (z) were detected. Y. enterocolitica was never isolated. Long chilling periods prior to wild game processing should be avoided, and carcasses should be tested at GHE rather than after shooting to proper reflect the microbial load of wild boar meat entering the food chain.



中文翻译:

野猪尸体的肠杆菌科细菌和沙门氏菌污染:不同采样策略之间的比较

2020 年期间,共对 64 头野猪尸体进行了腹部肠杆菌科细菌计数 (EBC)、沙门氏菌小肠结肠炎耶尔森氏菌检测 (i) 在狩猎采集点狩猎后 5 小时内,以及 (ii) 在屠宰场进行加工和加工之前。野味处理机构 (GHE)(49 头尸体——(i) 和 (ii) 之间的平均时间间隔:4.3 天)。由于 COVID-19 的限制,15 具尸体被运送到附近的屠宰场((i) 和 (ii) 之间的平均时间间隔:2.3 天)。收集肠系膜淋巴结(MLN)并检测沙门氏菌小肠结肠炎耶尔森氏菌。结果显示与采样 A(49 头屠体 - GHE)和采样 B(15 头屠体 - 屠宰场)相关。采样A:EBC中值为(i)2.51 log 10 CFU/cm 2和(ii)2.79 log 10 CFU/cm 2。(i) 和 (ii) 之间的 EBC 增加具有统计显着性 ( p  = 0.001)。屠体上沙门氏菌的患病率从 (i) 2.0 到 (ii) 6.1% 不等。采样B:EBC中值为(i)3.1 log 10 CFU/cm 2和(ii)3.32 log 10 CFU/cm 2。(i) 和 (ii) 之间的 EBC 增加没有统计学意义 ( p  = 0.191)。屠体上沙门氏菌的患病率从 (i) 6.7% 到 (ii) 0.0% 不等。淋巴沙门氏菌携带者的患病率(采样 A + B)为 7.8% (5/64)。从屠体和/或 MNL 中,检测到肠炎血清型、鼠伤寒血清型、Agama 血清型、Zaiman 血清型和 Diarizonae O:50 (z) 血清型。小肠结肠炎耶尔森氏菌从未被分离出来。应避免在野味加工前进行长时间的冷冻,并且应在 GHE 中对屠体进行测试,而不是在射击后进行测试,以正确反映进入食物链的野猪肉的微生物含量。

更新日期:2021-09-28
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