Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides,ACS Chemical Biology

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Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2021-09-27 , DOI: 10.1021/acschembio.1c00341
Meng Zheng ₁ , Maggie Zheng ₁ , Samuel Epstein ₁ , Alexa P Harnagel ₁ , Hanee Kim ₁ , Tania J Lupoli ₁

Affiliation

Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surface of the outer membrane. This review highlights chemical biology tools that have been developed in recent years to facilitate the modulation of O-antigen synthesis and composition, as well as related bacterial polysaccharide pathways, and the detection of unique glycan sequences. Advances in the biochemistry and structural biology of O-antigen biosynthetic machinery are also described, which provide guidance for the design of novel chemical and biomolecular probes. Many of the tools noted here have not yet been utilized in biological systems and offer researchers the opportunity to investigate the complex sugar architecture of Gram-negative cells.

中文翻译：

用于调节和可视化革兰氏阴性细菌表面多糖的化学生物学工具

细菌细胞呈现出多种多样的糖类，它们装饰细胞表面并帮助介导与环境的相互作用。许多革兰氏阴性细胞表达 O 抗原，O 抗原是构成外膜表面的脂多糖 (LPS) 远端部分的长糖聚合物。这篇综述重点介绍了近年来开发的化学生物学工具，以促进 O-抗原合成和组成的调节，以及相关的细菌多糖途径，以及独特聚糖序列的检测。还描述了 O 抗原生物合成机制的生物化学和结构生物学的进展, 为新型化学和生物分子探针的设计提供了指导。

更新日期：2021-10-15

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