In this study, phenolic compounds from an aqueous protein by-product from rapeseed meal (RSM) were identified by HPLC-DAD and HPLC-ESI-MS, including sinapine, sinapic acid, sinapoyl glucose, and 1,2-di-sinapoyl gentibiose. The main phenolic compound in this by-product was sinapine. We also performed acid hydrolysis to convert sinapine, and sinapic acid derivatives present in the permeate, to sinapic acid. The adsorption of phenolic compounds was investigated using five macroporous resins, including XAD4, XAD7, XAD16, XAD1180, and HP20. Among them, XAD16 showed the highest total phenolic contents adsorption capacities. The adsorption behavior of phenolic compounds was described by pseudo-second-order and Langmuir models. Moreover, thermodynamics tests demonstrated that the adsorption process of phenolic compounds was exothermic and spontaneous. The highest desorption ratio was obtained with 30% (v/v) and 70% (v/v) ethanol for sinapine and sinapic acid, respectively, with a desorption ratio of 63.19 ± 0.03% and 94.68 ± 0.013%. DPPH and ABTS tests revealed that the antioxidant activity of the hydrolyzed fraction was higher than the non-hydrolyzed fraction and higher than the one of vitamin C. Antioxidant tests demonstrated that these phenolic compounds could be used as natural antioxidants, which can be applied in the food industry.

中文翻译：

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大孔树脂从菜籽粕分离蛋白生产过程副产物中鉴定和捕获酚类化合物并评价其抗氧化性能

在本研究中，来自菜籽粕 (RSM) 的水性蛋白质副产品中的酚类化合物通过 HPLC-DAD 和 HPLC-ESI-MS 进行鉴定，包括芥子油、芥子酸、芥子油葡萄糖和 1,2-二芥子油龙胆二糖. 该副产品中的主要酚类化合物是芥子碱。我们还进行了酸水解以将存在于渗透物中的芥子酸和芥子酸衍生物转化为芥子酸。使用五种大孔树脂研究酚类化合物的吸附，包括 XAD4、XAD7、XAD16、XAD1180 和 HP20。其中，XAD16 表现出最高的总酚含量吸附能力。酚类化合物的吸附行为由拟二级模型和朗缪尔模型描述。此外，热力学测试表明酚类化合物的吸附过程是放热和自发的。v / v ) 和 70% ( v / v ) 乙醇分别用于芥子酸和芥子酸，解吸率分别为 63.19 ± 0.03% 和 94.68 ± 0.013%。DPPH 和 ABTS 测试表明，水解馏分的抗氧化活性高于非水解馏分，高于维生素 C 之一。抗氧化试验表明这些酚类化合物可作为天然抗氧化剂，可应用于食品工业。