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Overexpression approaches to advance understanding of Candida albicans
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-09-27 , DOI: 10.1111/mmi.14818
Laxmi Shanker Rai 1 , Lasse van Wijlick 1 , Murielle Chauvel 1 , Christophe d'Enfert 1 , Mélanie Legrand 1 , Sophie Bachellier-Bassi 1
Affiliation  

Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss-of-function approaches, taking advantage of gene knockouts or inducible down-regulation, have been successfully used in this species in order to understand gene function. However, overexpression of a gene provides an alternative, powerful tool to elucidate gene function and identify novel phenotypes. Notably, overexpression can identify pathway components that might remain undetected using loss-of-function approaches. Several repressible or inducible promoters have been developed which allow to shut off or turn on the expression of a gene in C. albicans upon growth in the presence of a repressor or inducer. In this review, we summarize recent overexpression approaches used to study different aspects of C. albicans biology, including morphogenesis, biofilm formation, drug tolerance, and commensalism.

中文翻译:

促进对白色念珠菌理解的过度表达方法

白色念珠菌是一种机会性真菌病原体,可导致与高死亡率相关的感染。利用基因敲除或诱导性下调的功能丧失方法已成功用于该物种,以了解基因功能。然而,基因的过表达提供了一种替代的、强大的工具来阐明基因功能和识别新的表型。值得注意的是,过表达可以识别使用功能丧失方法可能仍未检测到的途径成分。已经开发了几种可抑制或诱导型启动子,它们可以关闭或打开白色念珠菌中基因的表达在阻遏物或诱导物存在下生长。在这篇综述中,我们总结了最近用于研究白色念珠菌生物学不同方面的过表达方法,包括形态发生、生物膜形成、药物耐受性和共生性。
更新日期:2021-09-27
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