Lactic acid bacteria (LAB) naturally present on forage crops are responsible for the silage fermentation quality. However, there are few reports on the factors affecting the distribution of epiphytic LAB on the crops. Therefore, the influences of spraying sugars, growth temperatures and nitrogen application levels on the amounts and species of LAB on Italian ryegrass (Lolium multiflorum) were evaluated in this study. Spraying xylose, fructose, glucose, sucrose and galactose significantly increased (p < 0.05) the population sizes and species of LAB on Italian ryegrass. Spraying sucrose made Italian ryegrass epiphyte the most LAB. As for the effect of temperatures, Leuconostoc mesenteroides dominated on Italian ryegrass grown at 10 and 15°C, while Lactobacillus plantarum dominated at 20°C, and Weissella paramesenteroides dominated at 25°. The population sizes and species of LAB on Italian ryegrass were the most at 15°C. When N (0, 35, 70, 105, 140 or 175 kg/ha) was applied to Italian ryegrass, LAB population sizes and species were the most at N 35 kg/ha (p < 0.05). Lactobacillus plantarum was detected in all the treatments of Italian ryegrass except the growth temperatures of 10 and 25°C.

中文翻译：

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喷洒糖分、生长温度和施氮量改变了意大利黑麦草的附生乳酸菌组成

天然存在于饲草作物上的乳酸菌 (LAB) 对青贮饲料的发酵质量负责。然而，关于影响附生乳酸菌在作物上分布的因素的报道较少。因此，本研究评估了喷糖、生长温度和施氮水平对意大利黑麦草 ( Lolium multiflorum ) 上乳酸菌的数量和种类的影响。喷洒木糖、果糖、葡萄糖、蔗糖和半乳糖显着增加（p  < 0.05）意大利黑麦草上乳酸菌的数量和种类。喷洒蔗糖使意大利黑麦草附生植物成为最多LAB。至于温度的影响，Leuconostoc mesenteroides以 10 和 15°C 生长的意大利黑麦草为主，而植物乳杆菌在 20°C 占优势，Weissella paramesenteroides在 25°占优势。意大利黑麦草上 LAB 的种群规模和种类在 15°C 时最多。当 N（0、35、70、105、140 或 175 kg/ha）施用于意大利黑麦草时，LAB 种群规模和物种在 N 35 kg/ha 时最多（p  < 0.05）。除生长温度为 10 和 25°C 外，在意大利黑麦草的所有处理中均检测到植物乳杆菌。