The benefits of probiotics for the improvement of animal health status have been of great interest in recent years. For this reason, in this study was aimed at assessing a strain with probiotic potential to be added to the feed. Therefore, the objective of this trial is to use a strain with probiotic potential isolated from the intestinal microbiota of Helix aspersa Müller to subsequently add it to the feed of this species to improve its health status. So, the strain is characterized, and its probiotic potential is demonstrated. Finally, with the aim of preserving the probiotic strain by freeze-drying so that it can later be added to the feed, different cryoprotectants were studied that could give it a higher survival rate over time. The cryoprotectant that gives the best result with strain survival rate is trehalose 15%.

近年来，益生菌对改善动物健康状况的益处引起了人们的极大兴趣。因此，本研究旨在评估具有添加到饲料中的益生菌潜力的菌株。因此，本次试验的目的是利用从Helix aspersa Müller肠道微生物群中分离出的具有益生菌潜力的菌株，随后将其添加到该物种的饲料中，以改善其健康状况。因此，对该菌株进行了表征，并证明了其益生菌潜力。最后，为了通过冷冻干燥保存益生菌菌株，以便随后将其添加到饲料中，研究了不同的冷冻保护剂，以使其随着时间的推移具有更高的存活率。菌株存活率最好的冷冻保护剂是海藻糖15%。