We report the development of in vitro propagation protocols through an adventitious shoot induction pathway for a rare and medicinal Scutellaria havanensis. In vitro propagation studies using nodal explants showed MS medium supplemented with 10µM 6-Benzylaminopurine induced the highest number of adventitious shoots in a time-dependent manner. A ten-day incubation was optimum for shoot bud induction as longer exposures resulted in hyperhydricity of the explants and shoots induced. We also report preliminary evidence of Agrobacterium tumefaciens EHA105-mediated gene transfer transiently expressing the green fluorescent protein in this species. Transformation studies exhibited amenability of various explant tissues, internode being the most receptive. As the plant has medicinal value, research was carried out to evaluate its potential antioxidant capacity and the efficacy of methanolic leaf extracts in curbing the viability of human colorectal cancer cell line HCT116. Comparative total polyphenol and flavonoid content measurement of fresh and air-dried leaf extract revealed that the fresh leaf extracts contain higher total polyphenol and flavonoid content. The HCT 116 cell viability was assessed by colorimetric assay using a 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide, showed a steady growth inhibition after 24 h of incubation. Scanning electron microscopy of leaf surface revealed a high density of glandular and non-glandular trichomes. This research provides a basis for the conservation of this rare plant and future phytochemical screening and clinical research.

中文翻译：

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稀有黄芩的微繁殖 Jacq。抗氧化能力和抗癌潜力的初步研究

我们报告了通过稀有药用黄芩的不定枝诱导途径开发的体外繁殖协议。使用节点外植体的体外繁殖研究表明，添加 10µM 6-苄氨基嘌呤的 MS 培养基以时间依赖性方式诱导最多数量的不定芽。十天的孵化对于芽芽诱导是最佳的，因为较长的暴露导致外植体和诱导的芽的过度含水。我们还报告了根癌农杆菌的初步证据EHA105 介导的基因转移在该物种中瞬时表达绿色荧光蛋白。转化研究显示了各种外植体组织的适应性，节间是最容易接受的。由于该植物具有药用价值，因此进行了研究以评估其潜在的抗氧化能力和甲醇叶提取物在抑制人结肠直肠癌细胞系 HCT116 活力方面的功效。新鲜叶提取物和风干叶提取物的总多酚和类黄酮含量的比较测定表明，新鲜叶提取物含有较高的总多酚和类黄酮含量。HCT 116 细胞活力通过比色法使用 3-(4, 5-二甲基-噻唑-2-基)-2, 5-二苯基溴化四唑进行评估，在孵育 24 小时后显示稳定的生长抑制。叶表面的扫描电子显微镜显示高密度的腺毛和非腺毛。该研究为这种珍稀植物的保护以及未来的植物化学筛选和临床研究提供了依据。