A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 µL) was diluted (1:20) with a 7% (w/v) solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-C⁴D) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L l-histidine, 15 mmol/L dl-glutamic acid, and 30 µmol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-C⁴D analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-C⁴D method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma.

中文翻译：

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在运动员表现监测中使用毛细管电泳和非接触式电导检测对单滴血中的乳酸进行亚分钟分析

开发了一种简单快速的分析单滴血乳酸的方法。用 7% ( w / v ) 的 [三（羟甲基）甲基氨基] 丙磺酸溶液稀释（1:20）指尖刺全血样本（10 µL），并应用于血浆分离装置。该装置可容纳由不对称聚砜膜和支撑纺织膜组成的膜夹层，可在不到 20 秒的时间内将血浆收集到狭窄的玻璃毛细管中。分离并同时稀释的血浆直接注入带有非接触式电导检测器 (CE-C ⁴ D)的毛细管电泳仪中，并在不到一分钟的时间内进行分析。分离电解质由 10 mmol/L 组成l-组氨酸、15 mmol/L dl-谷氨酸和 30 µmol/L 十六烷基三甲基溴化铵。整个过程从手指点刺采样到 CE-C ⁴ D 分析完成，耗时不到 5 分钟，适用于监测增量自行车运动期间血浆中乳酸的增加。在通过开发的 CE-C ⁴ D 方法测量的实验中观察到的乳酸增加与手持式乳酸分析仪的结果 (R = 0.9882) 密切相关。开发的 CE 方法的优点是速度快、每次分析显着节省，并且可以分析血浆中的其他化合物。