α-glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme’s ligands present in Aloe vera, which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (2), together with two known compounds, aloenin aglycone (1) and aloin A (3), were fished out as the enzyme’s ligands. The structure of 2 was determined by HR-MS and comprehensive NMR techniques. Compound 3 exhibited a weak inhibitory effect on α-glucosidase, while compounds 1 and 2 were found to possess activation effects on the enzyme for the first time. It is interesting that both an inhibitor and agonists of α-glucosidase were fished out in one experiment.

中文翻译：

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α-葡萄糖苷酶功能化磁性纳米颗粒从芦荟中捕获α-葡萄糖苷酶的配体

α-葡萄糖苷酶固定在磁性纳米粒子 (MNP) 上，用于选择性固相提取芦荟中存在的酶配体，芦荟是一种用于治疗各种疾病并具有抗糖尿病活性的药用植物。一种新化合物 aloeacone ( 2 ) 与两种已知化合物 aloenin aglycone ( 1 ) 和aloin A ( 3 ) 一起作为酶的配体被提取出来。2的结构由HR-MS和综合核磁共振技术确定。化合物3对 α-葡萄糖苷酶的抑制作用较弱，而化合物1和2首次发现对酶具有活化作用。有趣的是，在一项实验中同时发现了 α-葡萄糖苷酶的抑制剂和激动剂。