Monochamus alternatus Hope (Coleoptera: Cerambycidae) is an important trunk borer of pine trees and a major vector of pine wilt disease. Although chemicals are widely used in forest pest control, new strategies based on insect biology are offering promising approaches to manage the disease. Although there have been important research advances in this respect, there has not yet been a deep sequence analysis of M. alternatus describing the transcriptome, and no information is available about the gene function of this insect vector. We used next generation sequencing technology to provide a full transcriptome from the four larval instars of M. alternatus and successfully built an M. alternatus transcriptome database. In total, 67,456 unigenes were obtained with trinity software, information for 11,858 classified unigenes was obtained with the Clusters of Orthologous Groups (COGs) database, and 13,007 unigenes matched predicted pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, genes related to lignocellulose, and putative Bt receptors and genes related to digestion are described. Additionally, the differential gene expression of these genes in different larval stages was analyzed. This study provides valuable information to underpin the development of new molecular tools for M. alternatus control strategies.

中文翻译：

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Monochamus alternatus Hope幼虫发育过程中基因表达阶段特异性变化的比较转录组分析

Monochamus alternatus Hope（鞘翅目：天牛科）是一种重要的松树树干螟虫，也是松树枯萎病的主要传播媒介。尽管化学品广泛用于森林病虫害防治，但基于昆虫生物学的新策略正在提供有希望的方法来控制疾病。虽然在这方面取得了重要的研究进展，但尚未对M. alternatus进行深入的描述转录组的序列分析，也没有关于这种昆虫载体基因功能的信息。我们使用新一代测序技术从M. alternatus的四个幼虫龄期提供完整的转录组，并成功构建了M. alternatus转录组数据库。使用 trinity 软件总共获得了 67,456 个 unigenes，通过直系同源群 (COG) 数据库获得了 11,858 个分类的 unigenes 信息，以及使用京都基因和基因组百科全书 (KEGG) 匹配预测通路的 13,007 个 unigenes。此外，还描述了与木质纤维素相关的基因，以及假定的 Bt 受体和与消化相关的基因。此外，还分析了这些基因在不同幼虫阶段的差异基因表达。这项研究提供了有价值的信息，以支持开发用于M.alternatus控制策略的新分子工具。