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Comparative analysis of chemical constituents by HPLC–ESI–MSn and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2021-09-26 , DOI: 10.1002/bmc.5250
Jingqiong Wan 1 , Xiaohui Gong 2 , Feixuan Wang 2, 3 , Chongwei Wen 2 , Yuan Wei 2 , Bangxing Han 4 , Zhen Ouyang 1, 2
Affiliation  

Dendrobium huoshanense is a Chinese medicinal herb that has high quality and excellent efficacy. However, the chemical basis of its activity is still unclear. Of note, Dendrobium officinale is the most widely utilized among the Dendrobium species. Therefore, the current study systematically investigated the chemical constituents of methanolic extracts and different polar fractions of aqueous extracts from the two herbs by HPLC–ESI–MSn, and then compared in vitro antioxidant activities of their five different polar extracts. Consequently, 61 and 49 compounds were identified from D. huoshanense and D. officinale, respectively, of which 43 compounds were common to both species. In addition, 17 out of 22 different compounds were identified only in D. huoshanense. Moreover, the peak areas of some shared identical compounds of D. huoshanense were significantly larger than that of D. officinale. In vitro antioxidant evaluation results showed that the n-BuOH-soluble fraction of the two herbs exhibited remarkable antioxidant activities. Furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale, which may be attributed to its variable and high contents of flavonoids, bibenzyls and phenanthrenes. These results provide the evidence for the high quality and efficacy of D. huoshanense.

中文翻译:

霍山石斛与铁皮石斛化学成分的HPLC-ESI-MSn比较分析及抗氧化活性

霍山石斛是一种品质优良、疗效极佳的中药材。然而,其活性的化学基础仍不清楚。值得注意的是,铁皮石斛是石斛种中应用最广泛的一种。因此,目前的研究通过 HPLC-ESI-MS n系统地研究了这两种草药的甲醇提取物和水提取物的不同极性部分的化学成分,然后比较了它们五种不同极性提取物的体外抗氧化活性。因此,从D. huoshanenseD. officinale中鉴定出 61 和 49 种化合物, 分别有 43 种化合物对这两个物种是共同的。此外,22 种不同化合物中的 17 种仅在D. hoshanense中鉴定。此外,霍山茶的一些相同化合物的峰面积明显大于铁皮。体外抗氧化评价结果表明,两种草药的丁醇可溶部分表现出显着的抗氧化活性。此外,霍山椹不同部位的抗氧化活性分别优于铁皮椴。,这可能是由于其类黄酮、联苄和菲的含量多变且含量高。这些结果为霍山枇杷的高质量和有效性提供了证据。
更新日期:2021-09-26
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