This experiment was designed to improve shoot proliferation by optimizing light intensity and reducing the potential effects of ethylene such as explant yellowing, unexpanded leaf and narrow shoots. For this purpose, shoot tips as explants were cultured in to the Murashige and Skoog medium with major salts at half‐strength supplemented with silver nanoparticles concentrations (0, 5, 10, 20, 40 µM AgNPs) which were then exposed to three different light intensities (40, 80, 160 µmol m⁻² s⁻¹) for a period of three months. Morphological (leaf number, dry and fresh weight of regenerants), biochemical and oxidant/antioxidant responses of regenerants were quantified. According to the results, low light intensity (40 µmol m⁻² s⁻¹) stimulated the shoot proliferation of Phalaenopsis and the effects of 5 µM AgNPs on shoot number while showing the maximum fresh and dry weight. It was also observed that chlorophyll, carbohydrate and protein content, enzymatic activity (superoxide dismutase and catalase), H₂O₂ generation, medium browning and phenolics content improve with higher light intensities (80 and 100 µmol m⁻² s⁻¹). Higher AgNP concentrations reduced shoot proliferation, dry weight, and soluble proteins. The most effective combinatorial treatment for the shoot proliferation stage of Phalaenopsis was determined to be 5 µM AgNPs and 40 µmol m⁻² s⁻¹.

该实验旨在通过优化光强度和减少乙烯的潜在影响（例如外植体变黄、未展开的叶子和狭窄的枝条）来改善枝条增殖。为此，将茎尖作为外植体培养到 Murashige 和 Skoog 培养基中，其中主要盐分半强度，并辅以银纳米粒子浓度（0、5、10、20、40 µM AgNPs），然后将其暴露在三种不同的光线下强度（40, 80, 160 µmol m ^-2 s ^-1）持续三个月。对再生剂的形态（叶数、干重和鲜重）、生化和氧化/抗氧化反应进行量化。根据结果​​，低光强度（40 µmol m ^-2 s ^-1) 刺激了蝴蝶兰的枝条增殖和 5 µM AgNPs 对枝条数量的影响，同时显示出最大的鲜重和干重。还观察到叶绿素、碳水化合物和蛋白质含量、酶活性（超氧化物歧化酶和过氧化氢酶）、H ₂ O ₂生成、中等褐变和酚类物质含量随着更高的光强度（80和100 μmol m ^-2 s ^-1）而改善。较高的 AgNP 浓度会降低枝条增殖、干重和可溶性蛋白质。蝴蝶兰枝条增殖阶段最有效的组合处理被确定为 5 µM AgNPs 和 40 µmol m ^-2 s ^-1。