Abstract

The study intends to probe the functions of miR-142-5p in retinoblastoma (RB) and the relationship between miR-142-5p and phosphatase and tensin homolog deleted on chromosome ten (PTEN). In our study, miR-142-5p and PTEN mRNA expression in RB tissue, serum of RB patients and RB cell lines were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, migration, invasion and cell apoptosis were measured using MTT assay, BrdU assay, Transwell experiments and flow cytometry analysis, respectively. Binding sites between miR-142-5p and PTEN were predicted by the TargetScan database and were confirmed via qRT-PCR, western blot and dual-luciferase reporter gene assay. It was demonstrated that miR-142-5p expression was elevated in RB tissue, serum of RB patients and RB cell lines. MiR-142-5p overexpression remarkably promoted the proliferation, migration, invasion and inhibited the apoptosis of WERI-RB-1 cells while miR-142-5p knockdown induced opposite effects in Y79 cells. MiR-142-5p decreased PTEN expression in both mRNA and protein expression levels, and PTEN was identified as a target gene of miR-142-5p. Cotransfection of PTEN overexpression plasmids reversed the influences of miR-142-5p on RB cells. In conclusion, miR-142-5p enhances proliferation, migration and invasion of RB cell by targeting PTEN.

中文翻译：

---

MiR-142-5p通过靶向PTEN促进视网膜母细胞瘤细胞增殖、迁移和侵袭

摘要

该研究旨在探讨miR-142-5p在视网膜母细胞瘤（RB）中的功能以及miR-142-5p与第十染色体（PTEN）上缺失的磷酸酶和张力蛋白同源物之间的关系。在我们的研究中，通过定量实时聚合酶链反应 (qRT-PCR) 研究了 RB 组织、RB 患者血清和 RB 细胞系中 miR-142-5p 和 PTEN mRNA 的表达。分别使用MTT法、BrdU法、Transwell实验和流式细胞术分析测量增殖、迁移、侵袭和细胞凋亡。miR-142-5p 和 PTEN 之间的结合位点由 TargetScan 数据库预测，并通过 qRT-PCR、蛋白质印迹和双荧光素酶报告基因测定进行确认。结果表明，在 RB 组织、RB 患者的血清和 RB 细胞系中，miR-142-5p 表达升高。MiR-142-5p 过表达显着促进 WERI-RB-1 细胞的增殖、迁移、侵袭并抑制细胞凋亡，而 miR-142-5p 敲低对 Y79 细胞产生相反的作用。MiR-142-5p 降低了 PTEN 的 mRNA 和蛋白质表达水平，PTEN 被鉴定为 miR-142-5p 的靶基因。PTEN 过表达质粒的共转染逆转了 miR-142-5p 对 RB 细胞的影响。总之，miR-142-5p通过靶向PTEN增强RB细胞的增殖、迁移和侵袭。PTEN 过表达质粒的共转染逆转了 miR-142-5p 对 RB 细胞的影响。总之，miR-142-5p通过靶向PTEN增强RB细胞的增殖、迁移和侵袭。PTEN 过表达质粒的共转染逆转了 miR-142-5p 对 RB 细胞的影响。总之，miR-142-5p通过靶向PTEN增强RB细胞的增殖、迁移和侵袭。