Field weed infestations can cause serious problems and require regular and planned programs to control them. Glyphosate is a broad-spectrum herbicide that inactivates the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) enzyme and causes plant death. It has been reported that the mutation of proline 101 to serine in EPSPS is one of the effective mutations to reduce the affinity of glyphosate to EPSPS enzyme. In this study, we investigated the effect of the bacterial P101S mutant aromatic acid (aroA) gene on glyphosate resistance in transgenic rapeseeds. For this purpose, the mutant gene was synthesized and cloned into the pUC18 and pBI121 vectors. The gene was transferred to rapeseed by the Agrobacterium-mediated method. In this experiment, three generations of transgenic plants (T0, T1, and T2) were studied under in vitro and in vivo conditions. After the treatment of 75 putative transgenic plants with 10 mM glyphosate in T0 generation, resistant plants were identified and their seeds were harvested. In the T1 generation, out of 200 cultivated plants, 141 showed resistance. After the plants were treated with herbicides and resistance was determined, the seeds were harvested when they mature. In the T2 generation, most plants (162 plants of 200) were resistant to glyphosate. Therefore, the inheritance of resistance followed Mendel's first law, which is a sign of the monogenic character of resistance. Purification and increasing the percentage of resistant plants will be carried out in the next generations. It is concluded that P101S mutation guarantees glyphosate resistance of rapeseed and is recommended to study it in other plants.

田间杂草侵扰可能会导致严重的问题，需要定期和有计划的计划来控制它们。草甘膦是一种广谱除草剂，可灭活 5-烯醇丙酮莽草酸 3-磷酸合酶 (EPSPS) 并导致植物死亡。据报道，EPSPS中脯氨酸101突变为丝氨酸是降低草甘膦与EPSPS酶亲和力的有效突变之一。在本研究中，我们研究了细菌 P101S 突变体芳香酸 ( aro A) 基因对转基因油菜籽草甘膦抗性的影响。为此，合成了突变基因并将其克隆到 pUC18 和 pBI121 载体中。通过农杆菌介导的方法将该基因转移到油菜籽中。在该实验中，在体外和体内条件下研究了三代转基因植物（T0、T1和T2）。在 T0 代用 10 mM 草甘膦处理 75 株假定的转基因植物后，鉴定出抗性植物并收获其种子。在 T1 代中，200 种栽培植物中，有 141 种显示出抗性。用除草剂处理植物并确定抗性后，在成熟时收获种子。在 T2 代中，大多数植物（200 株中有 162 株）对草甘膦具有抗性。因此，抗性的遗传遵循孟德尔第一定律，这是抗性单基因性的标志。纯化和增加抗性植物的百分比将在下一代进行。结论是P101S突变保证了油菜籽对草甘膦的抗性，建议在其他植物中进行研究。