Food allergy is a pathological condition that can lead to hives, swelling, gastrointestinal distress, cardiovascular and respiratory compromise, and even anaphylaxis. The lack of treatment resources emphasizes the necessity for new therapeutic strategies, and in this way, probiotics has been pointed out as an alternative, especially because of its immunomodulatory properties. The goal of this study was to evaluate the probiotic effect of Bifidobacterium longum subsp. longum 5^1A (BL5^1A) in a murine model of ovalbumin (OVA) food allergy, as well as to investigate the effect of the dose and viability of the bacteria on the proposed model. For this purpose, the probiotic effect was assessed by clinical, immunological, and histological parameters in mice treated or not with the BL5^1A and sensitized or not with OVA. Oral administration of BL5^1A prevented weight loss and reduced serum levels of IgE anti-OVA and of sIgA in the intestinal fluid. Also, it reduced the intestinal permeability, proximal jejunum damage, recruitment of eosinophils and neutrophils, and levels of eotaxin-1, CXCL1/KC, IL4, IL5, IL6, IL13, and TNF. Furthermore, the treatment was able to increase the levels of IL10. Investigating different doses administered, the level of 10⁸ CFU showed the best results in terms of protective effect. In addition, the administration of the inactivated bacteria did not present any beneficial effect. Results demonstrate that BL5^1A promotes a systemic immunomodulatory protective effect in a murine model of food allergy that depends on the dose and viability of the bacteria, suggesting its use as probiotic in such disease.

食物过敏是一种病理状况，可导致荨麻疹、肿胀、肠胃不适、心血管和呼吸系统受损，甚至过敏反应。治疗资源的缺乏强调了新治疗策略的必要性，因此，益生菌已被指出作为一种替代品，特别是因为它具有免疫调节特性。本研究的目的是评估长双歧杆菌亚种的益生菌作用。longum 5 ^1A (BL5 ^1A) 在卵清蛋白 (OVA) 食物过敏的小鼠模型中，以及研究细菌的剂量和活力对所提出模型的影响。为此，益生菌效应通过临床、免疫学和组织学参数在接受或未接受 BL5 ^1A治疗以及是否接受 OVA 致敏的小鼠中进行评估。口服 BL5 ^1A可防止体重减轻并降低肠液中 IgE 抗 OVA 和 sIgA 的血清水平。此外，它还降低了肠道通透性、近端空肠损伤、嗜酸性粒细胞和嗜中性粒细胞的募集以及嗜酸性粒细胞趋化因子-1、CXCL1/KC、IL4、IL5、IL6、IL13 和 TNF 的水平。此外，该治疗能够提高 IL10 的水平。^{调查不同给药剂量，10 8}的水平 CFU在保护作用方面表现出最好的结果。此外，灭活细菌的给药没有产生任何有益效果。结果表明，BL5 ^1A在食物过敏小鼠模型中促进全身免疫调节保护作用，这取决于细菌的剂量和活力，表明它在此类疾病中用作益生菌。