Transcriptome Analysis and Weighted Gene Co-expression Network Reveal Multitarget-Directed Antibacterial Mechanisms of Benzyl Isothiocyanate against Staphylococcus aureus,Journal of Agricultural and Food Chemistry

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Transcriptome Analysis and Weighted Gene Co-expression Network Reveal Multitarget-Directed Antibacterial Mechanisms of Benzyl Isothiocyanate against Staphylococcus aureus
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-24 , DOI: 10.1021/acs.jafc.1c03979
Hongbo Li ₁ , Xujia Ming ₁ , Dan Xu ₁ , Haizhen Mo ₁ , Zhenbin Liu ₁ , Liangbin Hu ₁ , Xiaohui Zhou ₂

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Staphylococcus aureus can cause many diseases and has a strong tendency to develop resistance to multiple antibiotics. In this study, benzyl isothiocyanate (BITC) was shown to have an excellent inhibitory effect on S. aureus ATCC25923 and methicillin-resistant S. aureus strains, with a minimum inhibitory concentration of 10 μg/mL. Under a scanning electron microscope, shrinkage and lysis of the cellular envelope were observed when exposed to BITC, and a bactericidal mode of BITC against S. aureus was further confirmed through flow cytometry. Additionally, the RNA profiles of S. aureus cells exposed to BITC indicated a violent transcriptional response to BITC. Through Kyoto Encyclopedia of Genes and Genomes analysis, it was found that many pathways involving bacterial survival were significantly affected, such as RNA degradation, oxidative phosphorylation, arginine biosynthesis, and so forth. A gene co-expression network was constructed using weighted gene co-expression network analysis, and six biologically meaningful co-expression modules and 125 hub genes were identified from the network. Among them, EfeB, GroES, SmpB, and Lsp were possibly targeted by BITC, leading to the death of S. aureus. Our results indicated a great potential of BITC to be applied in food safety and pharmaceuticals, highlighting its multitarget-directed bactericidal effects on S. aureus.

中文翻译：

转录组分析和加权基因共表达网络揭示异硫氰酸苄酯对金黄色葡萄球菌的多靶点抗菌机制

金黄色葡萄球菌可引起多种疾病，对多种抗生素有很强的耐药性。在本研究中，异硫氰酸苄酯 (BITC) 显示出对金黄色葡萄球菌ATCC25923 和耐甲氧西林金黄色葡萄球菌菌株具有极好的抑制作用，最小抑制浓度为 10 μg/mL。在扫描电镜下观察到暴露于 BITC 时细胞膜的收缩和裂解，并通过流式细胞术进一步证实了 BITC 对金黄色葡萄球菌的杀菌模式。此外，金黄色葡萄球菌的 RNA 谱暴露于 BITC 的细胞表明对 BITC 的剧烈转录反应。通过京都基因与基因组百科全书分析，发现许多涉及细菌存活的途径受到显着影响，如RNA降解、氧化磷酸化、精氨酸生物合成等。使用加权基因共表达网络分析构建基因共表达网络，并从网络中识别出六个具有生物学意义的共表达模块和125个枢纽基因。其中EfeB、GroES、SmpB和Lsp可能是BITC的目标，导致金黄色葡萄球菌死亡。我们的研究结果表明 BITC 在食品安全和药物方面具有巨大的应用潜力，突出了其对金黄色葡萄球菌的多靶点杀菌作用。

更新日期：2021-10-06

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