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New Insights into Stress-Induced β-Ocimene Biosynthesis in Tea (Camellia sinensis) Leaves during Oolong Tea Processing
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-23 , DOI: 10.1021/acs.jafc.1c04378
Si Chen 1, 2 , Peifeng Xie 1, 2 , Yeye Li 1, 2 , Xiaxia Wang 2 , Huihui Liu 1, 2 , Shanshan Wang 2 , Wenbo Han 1, 2 , Ruimei Wu 2 , Xinlei Li 1 , Yuefeng Guan 2 , Zhenbiao Yang 3 , Xiaomin Yu 2
Affiliation  

As the major contributors to the floral odors of tea products, terpenoid volatiles play critical roles in the defense response of plants to multiple stresses. Until now, only a few TPS genes in tea plants (Camellia sinensis) have been functionally validated. In this study, by comparative studies conducted at gene, protein, and metabolite levels during oolong tea processing, we isolated an ocimene synthase gene, CsOCS, which displays a low similarity to previously characterized tea ocimene synthases. Further prokaryotic expression and subcellular localization analysis showed that it is plastid-located and could produce (E)-β-ocimene and (Z)-β-ocimene using GPP as the substrate. The optimum temperature and pH of the enzyme were 30 °C and 7.5, respectively. Treatment with exogenous methyl jasmonate elevated the transcript level of CsOCS and enhanced the emission of ocimene from tea leaves. Collectively, CsOCS is implicated as a key enzyme for β-ocimene synthesis during oolong tea processing.

中文翻译:

对乌龙茶加工过程中茶(Camellia sinensis)叶中应激诱导的 β-罗勒烯生物合成的新见解

作为茶产品花香的主要贡献者,萜类挥发物在植物对多种胁迫的防御反应中起着关键作用。到目前为止,只有少数茶树 ( Camellia sinensis ) 中的TPS基因已被功能验证。在这项研究中,通过在乌龙茶加工过程中在基因、蛋白质和代谢物水平上进行的比较研究,我们分离了一个罗勒烯合酶基因CsOCS,它与先前表征的茶罗勒烯合酶具有较低的相似性。进一步的原核表达和亚细胞定位分析表明它位于质体并能产生( E )-β-罗勒烯和( Z)-β-罗勒烯使用 GPP 作为底物。该酶的最适温度和pH分别为30℃和7.5。用外源茉莉酸甲酯处理提高了CsOCS的转录水平并增强了茶叶中罗勒烯的排放。总的来说,CsOCS 被认为是乌龙茶加工过程中 β-罗勒烯合成的关键酶。
更新日期:2021-10-06
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