Irreversible electroporation (IRE) is an emerging tissue ablation technique with widespread potential, especially for cancer treatment. Although the safety and efficacy of IRE for gastric tissue ablation have been demonstrated, there is a gap of knowledge regarding the effect of electroporation pulse (EP) on the physiology and histopathology of the stomach. This study applied EP to the stomach of healthy rats and investigated the digestive function, serum marker levels, and gastric tissue structure of EP-treated rats. Ninety male rats were divided into nine groups and examined up to 28 days post-treatment. A single burst of electroporation pulse (500 V, 99 pluses, 1 Hz, 100 µs) was delivered to the stomachs of rats using a tweezer-style round electrode. Gastric emptying, small intestinal transit, and gastric secretion were measured to evaluate the digestive function. Serum marker levels were determined using ELISA. Haematoxylin–eosin, Masson trichrome, and immunofluorescence were performed for histopathological analysis. No significant effect on gastric emptying or secretion was found post-EP, whereas the small intestinal transit decreased at 4 h and rapidly recovered to normal on 1-day post-EP. Further, serum TNF-α and IL-1β levels temporarily changed during the acute phase but returned to baseline within 28 days. Moreover, histopathological analysis revealed that cell death occurred immediately post-EP in the ablation area, whereas the gastric wall scaffold in the ablation region remained intact post-EP. This study demonstrates the safety and efficacy of EP on the physiology and histopathology of the stomach and lays a foundation for more comprehensive applications of this technique.

中文翻译：

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电穿孔脉冲对大鼠胃的生理和组织病理学影响

不可逆电穿孔 (IRE) 是一种新兴的组织消融技术，具有广泛的潜力，尤其适用于癌症治疗。尽管已经证明了 IRE 用于胃组织消融的安全性和有效性，但关于电穿孔脉冲 (EP) 对胃生理学和组织病理学的影响仍存在知识空白。本研究将 EP 应用于健康大鼠的胃，并研究了 EP 治疗大鼠的消化功能、血清标志物水平和胃组织结构。九十只雄性大鼠被分成九组，并在治疗后长达 28 天进行检查。使用镊子式圆形电极将单次电穿孔脉冲 (500 V, 99 脉冲, 1 Hz, 100 µs) 传送到大鼠的胃中。胃排空，小肠转运，测定胃液分泌，评价消化功能。使用ELISA确定血清标志物水平。进行苏木精-伊红、马松三色和免疫荧光进行组织病理学分析。EP 后对胃排空或分泌没有显着影响，而小肠转运在 4 小时时减少，并在 EP 后 1 天迅速恢复正常。此外，血清 TNF-α 和 IL-1β 水平在急性期暂时改变，但在 28 天内恢复到基线水平。此外，组织病理学分析显示，消融区域的 EP 后立即发生细胞死亡，而消融区域的胃壁支架在 EP 后保持完整。