Ultraviolet/Visible and Near-Infrared Dual Spectroscopic Method for Detection and Quantification of Low-Level Malaria Parasitemia in Whole Blood,Analytical Chemistry

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Ultraviolet/Visible and Near-Infrared Dual Spectroscopic Method for Detection and Quantification of Low-Level Malaria Parasitemia in Whole Blood
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-09-24 , DOI: 10.1021/acs.analchem.1c02948
John A Adegoke ₁ , Amanda De Paoli ₂ , Isaac O Afara _{3,

4} , Kamila Kochan ₁ , Darren J Creek ₂ , Philip Heraud _{1,

5} , Bayden R Wood ₁

Affiliation

The scourge of malaria infection continues to strike hardest against pregnant women and children in Africa and South East Asia. For global elimination, testing methods that are ultrasensitive to low-level ring-staged parasitemia are urgently required. In this study, we used a novel approach for diagnosis of malaria infection by combining both electronic ultraviolet–visible (UV/vis) spectroscopy and near infrared (NIR) spectroscopy to detect and quantify low-level (1–0.000001%) ring-staged malaria-infected whole blood under physiological conditions uisng Multiclass classification using logistic regression, which showed that the best results were achieved using the extended wavelength range, providing an accuracy of 100% for most parasitemia classes. Likewise, partial least-squares regression (PLS-R) analysis showed a higher quantification sensitivity (R² = 0.898) for the extended spectral region compared to UV/vis and NIR (R² = 0.806 and 0.556, respectively). For quantifying different-stage blood parasites, the extended wavelength range was able to detect and quantify all thePlasmodium falciparum accurately compared to testing each spectral component separately. These results demonstrate the potential of a combined UV/vis–NIR spectroscopy to accurately diagnose malaria-infected patients without the need for elaborate sample preparation associated with the existing mid-IR approaches.

中文翻译：

紫外/可见光和近红外双光谱法检测和定量全血中的低水平疟原虫

疟疾感染的祸害继续对非洲和东南亚的孕妇和儿童造成最严重的打击。为了全球消除，迫切需要对低水平环形阶段寄生虫血症超敏感的测试方法。在这项研究中，我们通过结合电子紫外-可见 (UV/vis) 光谱和近红外 (NIR) 光谱来检测和量化低水平 (1–0.000001%) 环形阶段的疟疾感染，使用了一种新的方法来诊断疟疾感染。在生理条件下使用逻辑回归进行多类分类的受疟疾感染的全血，这表明使用扩展的波长范围可获得最佳结果，对大多数寄生虫血症类别提供 100% 的准确度。同样地，与 UV/vis 和 NIR（分别为R ² = 0.806 和 0.556）相比，扩展光谱区域的R ² = 0.898 )。为了量化不同阶段的血液寄生虫，与分别测试每个光谱成分相比，扩展的波长范围能够准确地检测和量化所有恶性疟原虫。这些结果证明了组合 UV/vis-NIR 光谱在准确诊断疟疾感染患者方面的潜力，而无需与现有的中红外方法相关的精细样品制备。

更新日期：2021-10-06

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