Mycotoxins have been widely studied by many research groups but further multidisciplinary research is needed to better understand and clarify many issues. This study describes the use of high-performance liquid chromatography coupled with ion trap mass spectrometry (HPLC-MS) to measure T-2 toxin and its metabolites, such as HT-2 toxin, neosolaniol (NEO) and diacetoxyscirpenol (DAS), as well as masked glucosylated mycotoxins in Fusarium-infected Czech spring barley. In total, 152 spring barley samples from the 2018 harvest were analyzed by the ELISA screening method for the presence of T-2 toxin. The most contaminated samples (15), which exceeded the recommended maximum level set by the EU for the sum of T-2 and HT-2 toxin in unprocessed cereals (200 μg/kg), were analyzed by HPLC-MS/MS and microbiological testing. Isolated fungi were evaluated microscopically and identified by polymerase chain reaction (PCR) assays. The prevalence of Fusarium species in spring barley across the Czech Republic in 2018 showed a predominance of F. poae (12 barley samples) and F. tricinctum (9 barley samples). Other strains (F. sporotrichioides and F. langsethiae) were present at a lower frequency, in 1 and 2 samples, respectively. The average concentration of T-2 plus HT-2 toxin was 107.7 μg/kg, while NEO and DAS were found in a few samples at values close to their limit of quantification. HT-2 glucoside was identified in all samples.

许多研究小组对霉菌毒素进行了广泛的研究，但需要进一步的多学科研究以更好地理解和澄清许多问题。本研究描述了使用高效液相色谱与离子阱质谱联用 (HPLC-MS) 来测量 T-2 毒素及其代谢物，如 HT-2 毒素、新茄醇 (NEO) 和二乙酰氧基scirpenol (DAS)，如以及镰刀菌中隐蔽的葡萄糖基化霉菌毒素- 受感染的捷克春季大麦。总共有 152 份来自 2018 年收获的春大麦样品通过 ELISA 筛选方法分析了 T-2 毒素的存在。污染最严重的样品 (15) 超过了欧盟为未加工谷物中 T-2 和 HT-2 毒素总和 (200 μg/kg) 设定的推荐最高水平，通过 HPLC-MS/MS 和微生物学分析测试。分离的真菌在显微镜下进行评估，并通过聚合酶链反应 (PCR) 测定进行鉴定。的患病率镰刀菌物种在2018横跨捷克共和国春大麦显示出占优势F. poae（12个大麦样本）和F. tricinctum（9个大麦样品）。其他菌株（F. sporotrichioides和F. langsethiae) 分别以较低的频率出现在 1 个和 2 个样本中。T-2 加 HT-2 毒素的平均浓度为 107.7 μg/kg，而在一些样品中发现 NEO 和 DAS 的值接近其定量限。在所有样品中都鉴定出 HT-2 糖苷。