The herbal plants contain various active compounds which have the ability to treat several human diseases without any side effects like cancer chemotherapeutic drugs used currently. It is reported that majority of the presently used drugs are derived from natural resources like plants or their products and hence, there is an urgent need to search for novel drugs from natural resources. Accordingly, the present study was designed to exploit an ethnomedicinally important plant Cassia auriculata Linn. for the identification of its phytoconstituents responsible for various properties such as antioxidant, anti-microbial and anti-diabetic activities. Different extracts of the plant were subjected to chromatographic techniques like high-performance liquid chromatography (HPLC), gas chromatography, and mass spectrometry (GC-MS) analysis to acquire the fingerprint of the phytoconstituents present in the plant. The extracts were analyzed by Ultra Violet-Visible (UV–Visible) and Fourier-Transform Infrared Spectroscopy (FT-IR). The efficacy of the extracts was examined through different in vitro assay methods. The GC-MS study exhibited the occurrence of 48 phytoconstituents in all the tested extracts and the HPLC study revealed the presence of quercetin. Different in vitro evaluations of the plant revealed that both ethyl acetate (DPPH-IC₅₀: 340.9 μg/ml) and ethanol fractions (DPPH-IC₅₀: 205.5 μg/ml) exhibited a potent activity. Hence, using the above study, novel potent antimicrobial and anti-diabetic principles from C. auriculata can be formulated in the future towards the clinical progress of therapeutic remedies against these ailments.

草本植物含有多种活性化合物，能够治疗多种人类疾病而没有任何副作用，如目前使用的癌症化疗药物。据报道，目前使用的药物大部分来源于植物或其制品等自然资源，因此迫切需要从自然资源中寻找新型药物。因此，本研究旨在开发一种在民族医学上具有重要意义的植物Cassia auriculata Linn。用于鉴定其负责各种特性的植物成分，例如抗氧化、抗微生物和抗糖尿病活性。对植物的不同提取物进行色谱技术，如高效液相色谱 (HPLC)、气相色谱和质谱 (GC-MS) 分析，以获得植物中存在的植物成分的指纹图谱。通过紫外-可见（UV-Visible）和傅里叶变换红外光谱（FT-IR）分析提取物。通过不同的体外测定方法检查提取物的功效。GC-MS 研究表明所有测试提取物中都存在 48 种植物成分，而 HPLC 研究表明存在槲皮素。对植物的不同体外评估表明，乙酸乙酯 (DPPH-IC ₅₀ : 340.9 μg/ml) 和乙醇组分 (DPPH-IC ₅₀: 205.5 μg/ml) 表现出有效的活性。因此，利用上述研究，可以在未来制定来自C. auriculata 的新型强效抗菌和抗糖尿病原理，以实现针对这些疾病的治疗方法的临床进展。