Osteosarcoma as an aggressive malignant neoplasm is one of the formidable challenging diseases clinically. The related tumor recurrence, large bone defects, and postoperative bacterial infection after surgical resection of osteosarcoma are pressing problems that still threaten patient health. Herein, a multiscale multifunctional theragenerative system (Fs-NFs-AuPt) is designed based on NiTi alloys for near-infrared-activated photothermal ablation of osteosarcoma, bone repair, and antibacterial. To achieve this aim, first, a useful methodology is proposed that employs temporally shaped femtosecond laser ablation combined with hydrothermal synthesis to fabricate multiscale hierarchical structures (Fs-NFs) consisting of three-dimensional micro-nanostructures (Fs-NiTi) and nanoflower-like structures (NFs) for promoting osteogenicity. Then, AuPt bimetallic nanoparticles as satisfactory photothermal therapy agents and osteoinductive agents are rapidly (only need 30 s) one-step in-situ synthesized on Fs-NFs through ultraviolet photoreduction without any reductant. Fs-NFs-AuPt significantly ablates tumors cells (Saos-2 and MDA-MB-231) in vitro, effectively inhibits osteosarcoma growth in mice, and positively induces the osteogenesis of osteoblasts (MC3T3-E1). Moreover, it exhibits superior antibacterial efficiencies of 99.5% and 99.8% against Staphylococcus aureus and Pseudomonas aeruginosa, respectively. Overall, this work offers a high-efficiency, high-quality, and high-repeatability distinctive integrated fabrication avenue to construct a multiscale multifunctional theragenerative system for osteosarcoma therapy and bone-tissue engineering.

骨肉瘤作为一种侵袭性恶性肿瘤，是临床上极具挑战性的疾病之一。骨肉瘤手术切除后相关的肿瘤复发、大骨缺损、术后细菌感染等问题仍然威胁着患者的健康。在此，基于 NiTi 合金设计了一种多尺度多功能治疗系统（Fs-NFs-AuPt），用于骨肉瘤的近红外光热消融、骨修复和抗菌。为了实现这一目标，首先，提出了一种有用的方法，它采用时间成形的飞秒激光烧蚀结合水热合成来制造由三维微纳米结构 (Fs-NiTi) 和纳米花状结构组成的多尺度分层结构 (Fs-NFs)。结构 (NFs) 以促进成骨性。然后，AuPt双金属纳米粒子作为令人满意的光热治疗剂和骨诱导剂，在没有任何还原剂的情况下，通过紫外光还原在Fs-NFs上快速（仅需要30秒）一步原位合成。Fs-NFs-AuPt 在体外显着消融肿瘤细胞（Saos-2 和 MDA-MB-231），有效抑制小鼠骨肉瘤的生长，并积极诱导成骨细胞（MC3T3-E1）的成骨。此外，它表现出 99.5% 和 99.8% 的卓越抗菌效率 并积极诱导成骨细胞 (MC3T3-E1) 的成骨。此外，它表现出 99.5% 和 99.8% 的卓越抗菌效率 并积极诱导成骨细胞 (MC3T3-E1) 的成骨。此外，它表现出 99.5% 和 99.8% 的卓越抗菌效率分别为金黄色葡萄球菌和铜绿假单胞菌。总体而言，这项工作提供了一条高效、高质量、高重复性的独特集成制造途径，以构建用于骨肉瘤治疗和骨组织工程的多尺度多功能治疗系统。