Abstract

This study was done of green synthesized zinc oxide nanoparticles (ZnO NPs) by using flaxseed extract (Linum usitatissimum L.) and investigated their potential anticancer effects. The ZnO NPs were characterized by UV–Vis, SEM/EDS, and XRD techniques. These analyses showed that these NPs were spherically shaped with a 23–55 nm size range. The cytotoxicity of NPs was confirmed by MTT assay against A549, HT29, and MDA-MB-231 cancer cells, indicating their antiproliferative effect (IC₅₀ values: 68.3, 66.3, and 58.8 μg/mL, respectively). The apoptotic induction was analyzed by ELISA, and the results showed that the NPs induced apoptosis through caspase-3/9 activation but not by caspase-8. These NPs significantly trigger the intrinsic-apoptotic pathway. Moreover, they exhibited strong antimigratory properties on the lowly metastatic HT29 and the highly metastatic A549 and MDA-MB-231 cells. This is the first report of Linum usitatissimum L-mediated synthesis of ZnO NPs that could be considered as potential antineoplastic agents.

摘要

这项研究是通过使用亚麻籽提取物 ( Linum usitatissimum L. )对绿色合成氧化锌纳米颗粒 (ZnO NPs) 进行的，并研究了它们的潜在抗癌作用。通过UV-Vis、SEM/EDS和XRD技术对ZnO纳米颗粒进行表征。这些分析表明，这些纳米颗粒呈球形，尺寸范围为 23-55 nm。MTT 法证实了 NPs 对 A549、HT29 和 MDA-MB-231 癌细胞的细胞毒性，表明它们的抗增殖作用（IC ₅₀值：分别为 68.3、66.3 和 58.8 μg/mL）。通过ELISA分析凋亡诱导，结果表明NPs通过caspase-3/9激活而不是caspase-8诱导细胞凋亡。这些 NPs 显着触发内源性凋亡途径。此外，它们对低转移性 HT29 和高转移性 A549 和 MDA-MB-231 细胞表现出强大的抗迁移特性。这是Linum usitatissimum L 介导的 ZnO NPs 合成的第一份报告，可将其视为潜在的抗肿瘤剂。