Abstract

A water-compatible molecularly imprinted polymer (MIP) was prepared for specific extraction of HIV-1 integrase inhibitor elvitegravir (EVG). It was prepared by a non-covalent free radical polymerization process using methacrylic acid as a monomer and elvitegravir as a template molecule. The MIP based solid phase extraction (MIP−SPE) cartridge was constructed for specific extraction of EVG from rat plasma samples. The effect of porogenic solvents, cross linker, pH and monomer to template ratio were studied. The developed HPLC method was validated as per ICH guidelines. The recovery of EVG using MIP−SPE technique was 98%. The LOD and LOQ of EVG were 0.01 and 0.05 µg/mL, respectively. The established method may not only be used to determine EVG but also to study the pharmacokinetics in rat plasma samples.

中文翻译：

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使用基于分子印迹聚合物的固相萃取在 RAT 血浆中对 Elvitegravir 进行 HPLC 生物测定：在药代动力学研究中的应用

摘要

制备了一种与水相容的分子印迹聚合物 ( MIP )，用于特异性提取 HIV-1 整合酶抑制剂 elvitegravir ( EVG )。它是通过非共价自由基聚合工艺制备的，以甲基丙烯酸为单体，elvitegravir 为模板分子。基于 MIP 的固相萃取 ( MIP-SPE) 小柱用于从大鼠血浆样品中特异性提取 EVG。研究了致孔溶剂、交联剂、pH 和单体与模板比例的影响。根据 ICH 指南对开发的 HPLC 方法进行了验证。使用 MIP-SPE 技术的 EVG 回收率为 98%。EVG 的 LOD 和 LOQ 分别为 0.01 和 0.05 µg/mL。所建立的方法不仅可用于确定 EVG，还可用于研究大鼠血浆样品中的药代动力学。