Abstract

A possibility of determining antibacterial preparations of the tetracycline series (tetracycline, doxycycline, oxytetracycline, demeclocycline, methacycline and chlortetracycline) in waste water, natural waters, and milk by the sensitized fluorescence of europium on its hydroxide using digital colorimetry (smartphone) is studied. It is found that europium(III) forms complexes with tetracyclines in an alkaline medium, and the formation of the europium hydroxide precipitate does not change its fluorescence properties. The colorimetric parameters of the precipitate after centrifugation are measured using a smartphone. The limits of detection and determination are 0.001–0.004 and 0.005–0.01 µg/mL, respectively, for all analytes. The analytical ranges are 0.005 (0.08)–0.1 (1) µg/mL. The calibration curves are linear, R² ≥ 0.98. A procedure for determining the total concentration of tetracyclines in waste water, natural waters, and milk is proposed. The duration of analysis is 20–30 min; the relative standard deviation of the results of analysis does not exceed 20%.

中文翻译：

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使用智能手机通过铕氢氧化物的敏化固态荧光测定水和牛奶中的四环素

摘要

研究了使用数字比色法（智能手机）通过铕对其氢氧化物的敏化荧光测定废水、天然水和牛奶中四环素系列（四环素、强力霉素、土霉素、地甲环素、甲环素和金霉素）抗菌制剂的可能性。发现铕(III)在碱性介质中与四环素形成络合物，氢氧化铕沉淀的形成不会改变其荧光特性。使用智能手机测量离心后沉淀物的比色参数。所有分析物的检测限和测定限分别为 0.001–0.004 和 0.005–0.01 µg/mL。分析范围为 0.005 (0.08)–0.1 (1) µg/mL。校准曲线是线性的，R² ≥0.98。提出了一种确定废水、天然水和牛奶中四环素总浓度的程序。分析持续时间为 20-30 分钟；分析结果的相对标准偏差不超过20%。